{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:43:56Z","timestamp":1792043036919,"version":"4.4.0"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u20102025\u201006282"],"award-info":[{"award-number":["RGPIN\u20102025\u201006282"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Journal"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Cannabis sativa<\/jats:italic>\n                    (\n                    <jats:italic>Cannabis<\/jats:italic>\n                    ) is a high\u2010value medicinal crop whose functional genomics and metabolic engineering remain constrained by limited genetic tools. The species is regarded as recalcitrant to stable transformation, and existing transient expression systems exhibit low efficiency or are restricted to hemp cultivars. Here, we report two\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    \u2010mediated agroinfiltration protocols enabling rapid, robust, and reproducible transient gene expression in drug\u2010type, high\u2010THC\n                    <jats:italic>Cannabis<\/jats:italic>\n                    tissues. Using the\n                    <jats:italic>RUBY<\/jats:italic>\n                    betalain reporter system, we demonstrate transformation efficiencies of up to 100% in mature leaf disks and 81%\u2013100% in whole seedlings. By independently comparing\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    strains and vector configurations, we demonstrate that transformation efficiency and transgene expression are influenced by distinct experimental variables, with EHA105 providing the highest transformation efficiency and\n                    <jats:italic>p35S<\/jats:italic>\n                    \u2010based vectors producing the strongest reporter expression. Complementing these genetic tools, we developed a UPLC\u2010PDA method enabling simultaneous detection and quantification of cannabinoids and cannflavins. While not intended to replace mass spectrometry\u2010based analyses, the method provides an accessible and cost\u2010effective platform for comparative metabolite profiling during functional genomics and metabolic engineering studies. Together, these advances establish an integrated workflow linking high\u2010efficiency transient gene expression with streamlined biochemical analysis, providing a practical foundation for gene function studies, pathway interrogation, and biotechnology\u2010driven trait engineering in\n                    <jats:italic>Cannabis<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/biot.70316","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T04:43:59Z","timestamp":1792039439000},"source":"Crossref","is-referenced-by-count":0,"title":["An Integrated Transient Expression and UPLC\u2010PDA Approach for Functional Genomics and Metabolic Analysis of\n                    <i>Cannabis sativa<\/i>"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9854-0023","authenticated-orcid":false,"given":"Sarah","family":"Pullano","sequence":"first","affiliation":[{"name":"Department of Biology York University  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2142-5385","authenticated-orcid":false,"given":"Aida","family":"Shahabi","sequence":"additional","affiliation":[{"name":"Department of Biology York University  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8462-7842","authenticated-orcid":false,"given":"Parmis Shariat","family":"Torbaghani","sequence":"additional","affiliation":[{"name":"Department of Biology York University  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9709-0830","authenticated-orcid":false,"given":"Ivan","family":"Monsalvo","sequence":"additional","affiliation":[{"name":"Department of Biology York University  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madison","family":"Sandler","sequence":"additional","affiliation":[{"name":"Department of Biology York University  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4745-3305","authenticated-orcid":false,"given":"Adam","family":"Sumner","sequence":"additional","affiliation":[{"name":"College of Agricultural and Life Sciences Virginia Tech  Blacksburg Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3120-655X","authenticated-orcid":false,"given":"Alex","family":"Harris","sequence":"additional","affiliation":[{"name":"College of Agricultural and Life Sciences Virginia Tech  Blacksburg Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3692-3152","authenticated-orcid":false,"given":"David","family":"Haak","sequence":"additional","affiliation":[{"name":"College of Agricultural and Life Sciences Virginia Tech  Blacksburg Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8406-5036","authenticated-orcid":false,"given":"Bastiaan","family":"Bargmann","sequence":"additional","affiliation":[{"name":"College of Agricultural and Life Sciences Virginia Tech  Blacksburg Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9396-6626","authenticated-orcid":false,"given":"Nik","family":"Kovinich","sequence":"additional","affiliation":[{"name":"Department of Biology York University  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.10.039"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhad150"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcab.2024.103250"},{"key":"e_1_2_11_5_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/07060661.2026.2649769","article-title":"Hop Latent Viroid\u2010Infected Cannabis (Cannabis sativa L.) 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