{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:08:15Z","timestamp":1762250895592},"reference-count":62,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,3,13]],"date-time":"2003-03-13T00:00:00Z","timestamp":1047513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biopolymers"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human islet amyloid polypeptide (hIAPP), or amylin, is a 37 amino acid hormone secreted by pancreatic \u03b2\u2010cells. hIAPP constitutes \u223c90% of the amyloid deposits found in type II diabetic patients. It has been shown that the central region of the peptide (hIAPP<jats:sub>20\u201329<\/jats:sub>) constitutes the nucleation site for the amyloidogenic process with F23 playing a key role in the formation of the \u03b2\u2010pleated structures. In addition, it has been proposed that an important stage in the cytotoxicity of hIAPP is its interaction with the \u03b2\u2010cell membranes. As a first step toward the characterization of the interaction of hIAPP with cell membranes, we determined conformational preferences of hIAPP<jats:sub>20\u201329<\/jats:sub>in membrane\u2010mimicking environments. We found that upon interacting with negatively charged micelles, the dominant conformation of hIAPP<jats:sub>20\u201329<\/jats:sub>is a distorted type I \u03b2\u2010turn centered on residues F23 and G24, with F23, A25, and I26 forming a small hydrophobic cluster that may facilitate the interaction of this peptide with the membrane bilayer. Moreover, we were able to elucidate the topological orientation of the peptide that is absorbed on the micelle surface, with the hydrophobic cluster oriented toward the hydrocarbon region of the micelles and both N\u2010 and C\u2010termini exposed to the solvent. \u00a9 2003 Wiley Periodicals, Inc. Biopolymers 69: 29\u201341, 2003<\/jats:p>","DOI":"10.1002\/bip.10305","type":"journal-article","created":{"date-parts":[[2003,4,22]],"date-time":"2003-04-22T10:01:40Z","timestamp":1051005700000},"page":"29-41","source":"Crossref","is-referenced-by-count":58,"title":["Conformational preferences of the amylin nucleation site in SDS micelles: An NMR study"],"prefix":"10.1002","volume":"69","author":[{"given":"Alessandro","family":"Mascioni","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Porcelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Udayar","family":"Ilangovan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayyalusamy","family":"Ramamoorthy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianluigi","family":"Veglia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,3,13]]},"reference":[{"key":"e_1_2_7_2_2","first-page":"8638","volume":"84","author":"Cooper G. J. S.","year":"1987","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/340272b0"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-15-2-163"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.1163"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1042\/bst0230325"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(81)80197-9"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01219652"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00076a078"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1998.2510208.x"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.2646"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.9.2.427"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.4.1988"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.13.5036"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(92)90411-6"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3422"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi981658g"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M102883200"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00117a023"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00028a073"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(89)90407-2"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.39.1.118"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(88)80538-2"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(99)00248-4"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(80)90695-6"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02192855"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(85)90018-6"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(88)90178-3"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00197809"},{"key":"e_1_2_7_30_2","volume-title":"SPARKY 3","author":"Goddard T. D.","year":"1999"},{"key":"e_1_2_7_31_2","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1093\/oso\/9780199632251.001.0001","volume-title":"NMR of Macromolecules","author":"Roberts G. C. K.","year":"1993"},{"key":"e_1_2_7_32_2","unstructured":"Brunger A. T.X\u2010Plor Version 3.1. A System for X\u2010ray Crystallography and NMR 1992."},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/2.1.27"},{"key":"e_1_2_7_34_2","first-page":"477","volume":"8","author":"Laskowski R. A.","year":"1998","journal-title":"J Biomol NMR"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(01)39318-7"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.66.1.385"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.15.7942"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bb.20.060191.002511"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M102883200"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1985.tb09096.x"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(88)90103-9"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/3.6.479"},{"key":"e_1_2_7_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0282(199801)45:1<9::AID-BIP2>3.0.CO;2-X"},{"key":"e_1_2_7_44_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.6.1989"},{"key":"e_1_2_7_45_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2000.4887"},{"issue":"4","key":"e_1_2_7_46_2","first-page":"240","volume":"4","author":"Jao S. C.","year":"1997","journal-title":"Int J Exp Clin Invest"},{"key":"e_1_2_7_47_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja990864o"},{"key":"e_1_2_7_48_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja9738687"},{"issue":"2","key":"e_1_2_7_49_2","first-page":"A299","volume":"74","author":"Shao H.","year":"1998","journal-title":"Biophys J"},{"key":"e_1_2_7_50_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.2348"},{"key":"e_1_2_7_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(99)09015-1"},{"issue":"2","key":"e_1_2_7_52_2","first-page":"A220","volume":"74","author":"Ma K.","year":"1998","journal-title":"Biophys J"},{"issue":"1","key":"e_1_2_7_53_2","first-page":"667","volume":"80","author":"Kirkitadze M. D.","year":"2001","journal-title":"Biophys J"},{"key":"e_1_2_7_54_2","doi-asserted-by":"publisher","DOI":"10.1021\/tx970009n"},{"key":"e_1_2_7_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(94)39020-7"},{"key":"e_1_2_7_56_2","first-page":"845","volume":"4","author":"Opella S. J.","year":"1997","journal-title":"Nat Struct Biol"},{"key":"e_1_2_7_57_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.2000.4288"},{"key":"e_1_2_7_58_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00083a036"},{"key":"e_1_2_7_59_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.0540h.x"},{"key":"e_1_2_7_60_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.2000.4267"},{"key":"e_1_2_7_61_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C000483200"},{"key":"e_1_2_7_62_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.3980"},{"key":"e_1_2_7_63_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi020314u"}],"container-title":["Biopolymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbip.10305","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bip.10305","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T19:22:44Z","timestamp":1704828164000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bip.10305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,3,13]]},"references-count":62,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,5]]}},"alternative-id":["10.1002\/bip.10305"],"URL":"https:\/\/doi.org\/10.1002\/bip.10305","archive":["Portico"],"relation":{},"ISSN":["0006-3525","1097-0282"],"issn-type":[{"value":"0006-3525","type":"print"},{"value":"1097-0282","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,3,13]]}}}