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Two of them (Thr\u2010362 and Ser\u2010366) are located within GSGKGKVQFQGKKTKFASDD (346\u2013368) and one (Thr\u2010302) within VTWEVLEGEVEKEALKKI (301\u2013318), which are main B\u2010cell epitopes of La\/SSB. With the aim to investigate how phosphorylation, one of the most common posttranslational protein modifications, affects the antigenic and conformational characteristics of the La\/SSB epitopes, we synthesized and studied the phosphorylated epitopes La\/SSB(346\u2013368)\u2010P, La\/SSB(359\u2013368)\u2010P, and La\/SSB(301\u2013318)\u2010P with respect to their nonphosphorylated counterparts. Anti\u2010La\/SSB positive sera from SS and SLE patients are better recognized by the phosphorylated epitopes compared to their nonphosphorylated counterparts. Conformational analysis by<jats:sup>1<\/jats:sup>H nuclear magnetic resonance spectroscopy and molecular dynamics showed that the phosphorylated epitopes adopt different structural characteristics from those of the corresponding nonphosphorylated epitopes. It is concluded that phosphorylation can create neoepitopes with altered functions, compared to the nonphosphorylated epitopes, which might be seen from the immune system as \u201cforeign.\u201d \u00a9 2006 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 84: 368\u2013382, 2006<\/jats:p><jats:p>This article was originally published online as an accepted preprint. The \u201cPublished Online\u201d date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com<\/jats:p>","DOI":"10.1002\/bip.20458","type":"journal-article","created":{"date-parts":[[2006,2,2]],"date-time":"2006-02-02T11:46:28Z","timestamp":1138880788000},"page":"368-382","source":"Crossref","is-referenced-by-count":7,"title":["Phosphorylated and nonphosphorylated epitopes of the La\/SSB autoantigen: Comparison of their antigenic and conformational characteristics"],"prefix":"10.1002","volume":"84","author":[{"given":"Nicolas","family":"Coudevylle","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios","family":"Rokas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Sakarellos\u2010Daitsiotis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios","family":"Krikorian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenia","family":"Panou\u2010Pomonis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constantinos","family":"Sakarellos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Boussard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manh Thong","family":"Cung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,2,2]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"3744","DOI":"10.4049\/jimmunol.136.10.3744","volume":"136","author":"Chan E. 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