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Though the real pathogenesis is still unknown, many investigations have revealed that conformational alteration and fibril formation of \u03b1\u2010Syn protein have an important role in causing the disease. In this work, we introduced the <jats:italic>g<\/jats:italic>\u2010factor spectra of solid\u2010state circular dichroism to estimate the secondary structure contents of \u03b1\u2010Syn fragments in amyloids. Fourier\u2010transform infrared (FTIR) was also applied to confirm the structural formation. The results suggest that the central hydrophobic region is critical for \u03b2\u2010sheet formation and the conformational alteration is the foundation of protein abnormal aggregation. The research provides a practical approach to estimate the secondary structure contents of protein amyloids and further insight into the relevance of structural transformation and amyloidogenesis. \u00a9 2006 Wiley Periodicals, Inc. Biopolymers 83: 226\u2013232, 2006<\/jats:p><jats:p>This article was originally published online as an accepted preprint. The \u201cPublished Online\u201d date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com<\/jats:p>","DOI":"10.1002\/bip.20550","type":"journal-article","created":{"date-parts":[[2006,6,2]],"date-time":"2006-06-02T20:29:27Z","timestamp":1149280167000},"page":"226-232","source":"Crossref","is-referenced-by-count":19,"title":["Secondary structural formation of \u03b1\u2010synuclein amyloids as revealed by <i>g<\/i>\u2010factor of solid\u2010state circular dichroism"],"prefix":"10.1002","volume":"83","author":[{"given":"Xiao\u2010Jing","family":"Lin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan\u2010Yuan","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Jing","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Hua","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Yu","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,6,2]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1067122"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(99)00049-4"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.18.9989"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2003.12.025"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/00019052-200108000-00001"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(97)01213-7"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-5521(95)90071-3"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi034028"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(95)46006-3"},{"key":"e_1_2_6_11_2","first-page":"129","volume":"299","author":"Calero M.","year":"2005","journal-title":"Methods Mol Biol"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0282(2001)62:1<15::AID-BIP30>3.0.CO;2-J"},{"key":"e_1_2_6_13_2","first-page":"445","volume":"8","author":"Kuhn W.","year":"1930","journal-title":"Physik Chem Abt B"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2001.5113"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsb961"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/bip.20095"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2005.07.008"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/bip.10179"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.05.050"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi048448q"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi020429y"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.05025.x"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.2174\/092986606775338470"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10930-005-6713-4"},{"key":"e_1_2_6_25_2","first-page":"393","volume":"13","author":"Li H. 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