{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:09:45Z","timestamp":1760954985302},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,6,4]],"date-time":"2007-06-04T00:00:00Z","timestamp":1180915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biopolymers"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sheep fasciolosis is a devastating burden for the livestock industry. We herein report on immunodiagnosis of fasciolosis, and significant protection of sheep against challenge infection with <jats:italic>Fasciola gigantica<\/jats:italic> following immunization with a peptide based on the H\u2010Asp<jats:sup>110<\/jats:sup>\u2010Lys\u2010Ile\u2010Asp\u2010Trp\u2010Arg\u2010Glu\u2010Ser\u2010Gly\u2010Tyr\u2010Val\u2010Thr\u2010Glu\u2010Val<jats:sup>123<\/jats:sup>\u2010OH (Fas14p) sequence of <jats:italic>F. gigantica<\/jats:italic> cathepsin L\u2010cysteine proteinase. This sequence was synthesized in three different forms: as N<jats:sup>\u03b1<\/jats:sup> acetylated (Ac\u2010Asp<jats:sup>110<\/jats:sup>\u2010Lys\u2010Ile\u2010Asp\u2010Trp\u2010Arg\u2010Glu\u2010Ser\u2010Gly\u2010Tyr\u2010Val\u2010Thr\u2010Glu\u2010Val<jats:sup>123<\/jats:sup>\u2010OH, FasAc14p), bearing at the amino\u2010terminus an N<jats:sup>\u03b1<\/jats:sup> acetylated cystein (Ac\u2010Cys\u2010Asp<jats:sup>110<\/jats:sup>\u2010Lys\u2010Ile\u2010Asp\u2010Trp\u2010Arg\u2010Glu\u2010Ser\u2010Gly\u2010Tyr\u2010Val\u2010Thr\u2010Glu\u2010Val<jats:sup>123<\/jats:sup>\u2010OH, FasAcCys14p), and conjugated to sequential oligopeptide carrier Ac\u2010[Lys\u2010Aib\u2010Gly]<jats:sub>4<\/jats:sub>\u2010OH (Ac\u2010SOC<jats:sub>4<\/jats:sub>) through an amide bond formed between Val<jats:sup>123<\/jats:sup> carboxylic group of the epitope and the lysine N<jats:sup>\u03f5<\/jats:sup> groups of the carrier (Ac\u2010[Lys(Fas14p)\u2010Aib\u2010Gly]<jats:sub>4<\/jats:sub>\u2010OH). Ac\u2010[Lys(Fas14p)\u2010Aib\u2010Gly]<jats:sub>4<\/jats:sub>\u2010OH failed to readily discriminate between na\u00efve and infected sheep. In contrast, the free peptides reproducibly differentiated between parasite\u2010free sheep, sheep infected with parasites other than <jats:italic>Fasciola<\/jats:italic>, and experimentally <jats:italic>Fasciola<\/jats:italic>\u2010infected sheep. The data together indicated that the peptides might be of considerable use for discriminating between early and late, and low and high burden, sheep infection with <jats:italic>F. gigantica<\/jats:italic>. FasAc14p was chosen to determine whether a peptide based on a critical enzymatic site of cathepsin L proteinase may induce protection against challenge infection. Sheep immunization with FasAc14p peptide induced significant expression of interleukin\u20104 mRNA, and humoral antibodies that bound to molecule(s) on the intact surface membrane of newly excysted juvenile worms, and mediated their attrition. The immune responses were associated with significant (<jats:italic>P<\/jats:italic> &lt; 0.02) decrease of 23.1% in worm recovery, but with no decrease in the size or maturation of worms recovered. \u00a9 2007 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 90:349\u2013357, 2008.<\/jats:p><jats:p>This article was originally published online as an accepted preprint. The \u201cPublished Online\u201d date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com<\/jats:p>","DOI":"10.1002\/bip.20788","type":"journal-article","created":{"date-parts":[[2007,6,4]],"date-time":"2007-06-04T16:19:15Z","timestamp":1180973955000},"page":"349-357","source":"Crossref","is-referenced-by-count":16,"title":["<i>Fasciola gigantica<\/i> cathepsin L proteinase\u2010based synthetic peptide for immunodiagnosis and prevention of sheep fasciolosis"],"prefix":"10.1002","volume":"90","author":[{"given":"Jan","family":"Jezek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rashika El","family":"Ridi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"Salah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amal","family":"Wagih","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haidy W.","family":"Aziz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hatem","family":"Tallima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed H.","family":"El Shafie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarek Abdel","family":"Khalek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faten F.","family":"Abo Ammou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Constantinos","family":"Strongylis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vassilios","family":"Moussis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vassilios","family":"Tsikaris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,4,21]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"104","volume-title":"In Handbook on Animal Diseases in the Tropics","author":"Hammond J. 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