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The most efficient synthesis used double and triple couplings and 10 equiv. of amino acids. Double couplings were used for most amino acids, whereas triple couplings were utilized for amino acids in selected regions. The most effective method for formation of the disulfide bond in amylin was found to be iodine oxidation. The highest purity amylin was obtained when the crude peptide was purified with HPLC before formation of the disulfide bond. The cytotoxicity of the synthesized amylin product to RIN\u20105F cells was determined. The synthesized amylin exhibits an exponential increase of cytotoxicity at concentrations &gt;35 \u03bc<jats:italic>M<\/jats:italic>. Transmission electron microscope studies of a sample of amylin shows that insoluble amyloid fibrils spontaneously formed when 45 \u03bc<jats:italic>M<\/jats:italic> solution of synthesized amylin was incubated in a suitable buffer for 6 h. \u00a9 2010 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 94: 323\u2013330, 2010.<\/jats:p><jats:p>This article was originally published online as an accepted preprint. The \u201cPublished Online\u201d date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com<\/jats:p>","DOI":"10.1002\/bip.21370","type":"journal-article","created":{"date-parts":[[2010,1,12]],"date-time":"2010-01-12T19:19:10Z","timestamp":1263323950000},"page":"323-330","source":"Crossref","is-referenced-by-count":18,"title":["Microwave assisted SPPS of amylin and its toxicity of the pure product to RIN\u20105F cells"],"prefix":"10.1002","volume":"94","author":[{"given":"Karen","family":"Muthusamy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Albericio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Per I.","family":"Arvidsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Govender","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hendrik G.","family":"Kruger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glenn E. 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