{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T17:59:35Z","timestamp":1769968775185,"version":"3.49.0"},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2010,6,23]],"date-time":"2010-06-23T00:00:00Z","timestamp":1277251200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biopolymers"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The tyrosine aminomutase <jats:italic>Sg<\/jats:italic>TAM produces (<jats:italic>S<\/jats:italic>)\u2010\u00df\u2010tyrosine from <jats:sc>L<\/jats:sc>\u2010tyrosine in the biosynthesis of the enediyne antitumor antibiotic C\u20101027. This conversion is promoted by the methylideneimidazole\u20105\u2010one (MIO) prosthetic group. MIO was first identified in the homologous family of ammonia lyases, which deaminate aromatic amino acids to form \u03b1,\u00df\u2010unsaturated carboxylates. Studies of substrate specificity have been described for lyases but there have been limited reports in altering the substrate specificity of aminomutases. Furthermore, it remains unclear as to what structural properties are responsible for catalyzing the presumed readdition of the amino group into the \u03b1,\u00df\u2010unsaturated intermediates to form \u00df\u2010amino acids. Attempts to elucidate specificity and mechanistic determinants of <jats:italic>Sg<\/jats:italic>TAM have also proved to be difficult as it is recalcitrant to perturbations to the active site via mutagenesis. An X\u2010ray cocrystal structure of the <jats:italic>Sg<\/jats:italic>TAM mutant of the catalytic base with <jats:sc>L<\/jats:sc>\u2010tyrosine verified important substrate binding residues as well as the enzymatic base. Further mutagenesis revealed that removal of these crucial interactions renders the enzyme inactive. Proposed structural determinants for mutase activity probed via mutagenesis, time\u2010point assays and X\u2010ray crystallography revealed a complicated role for these residues in maintaining key quaternary structure properties that aid in catalysis. \u00a9 2010 Wiley Periodicals, Inc. Biopolymers 93: 802\u2013810, 2010.<\/jats:p>","DOI":"10.1002\/bip.21500","type":"journal-article","created":{"date-parts":[[2010,6,24]],"date-time":"2010-06-24T05:56:39Z","timestamp":1277358999000},"page":"802-810","source":"Crossref","is-referenced-by-count":27,"title":["Probing the active site of MIO\u2010dependent aminomutases, key catalysts in the biosynthesis of \u03b2\u2010amino acids incorporated in secondary metabolites"],"prefix":"10.1002","volume":"93","author":[{"given":"Heather A.","family":"Cooke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven D.","family":"Bruner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,6,23]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2009.06.013"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi982929q"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1367-5931(00)00253-2"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200461377"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi035223r"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja034609m"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411215200"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M703439200"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/cb800085g"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200800748"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja073275o"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00333a023"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi049053"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2006.11.011"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2008.05.025"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi061774g"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi7003685"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2007.11.046"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0762689"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06068"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi700691p"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2008.02.007"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2006.10.008"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889897006766"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.406"}],"container-title":["Biopolymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbip.21500","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bip.21500","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T03:04:39Z","timestamp":1694401479000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bip.21500"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6,23]]},"references-count":26,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["10.1002\/bip.21500"],"URL":"https:\/\/doi.org\/10.1002\/bip.21500","archive":["Portico"],"relation":{},"ISSN":["0006-3525","1097-0282"],"issn-type":[{"value":"0006-3525","type":"print"},{"value":"1097-0282","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,6,23]]}}}