{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T16:21:41Z","timestamp":1780676501844,"version":"3.54.1"},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,9,20]],"date-time":"2010-09-20T00:00:00Z","timestamp":1284940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biopolymers"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\u03b1\u2010Crystallin, the major protein of mammalian eye lens, is a member of the small heat shock protein family and is a molecular chaperone. We previously reported that its molecular chaperone function as well as stability increased in presence of Zn<jats:sup>+2<\/jats:sup>. Despite the effect of Zn<jats:sup>+2<\/jats:sup> on the structure and function of \u03b1\u2010crystallin, evidence for direct interaction between them remained elusive. We now present the MALDI mass spectrometric data that shows direct evidence of Zn<jats:sup>+2<\/jats:sup> binding to recombinant \u03b1A\u2010 and \u03b1B\u2010crystallin. The binding stoichiometry was over three Zn<jats:sup>+2<\/jats:sup> per subunit of \u03b1\u2010crystallin at zinc\/protein molar ratio of 20. Observation of multiple Zn<jats:sup>+2<\/jats:sup> binding is consistent with the large increase in thermodynamic stability. Sequence\u2010based analysis of \u03b1A\u2010 and \u03b1B\u2010crystallin predicted both proteins to be nonzinc binding proteins. Our dynamic light scattering data shows that Zn<jats:sup>+2<\/jats:sup> stabilizes the oligomeric structure of \u03b1\u2010crystallin by bridging neighboring subunits in multiple centers. Despite the low affinity binding, the intersubunit bridging by multiple Zn<jats:sup>+2<\/jats:sup> makes the oligomer so stable that oligomer breakdown does not occur even at 6<jats:italic>M<\/jats:italic> urea. The subunit bridging has been supported by our FRET data that showed absence of subunit exchange in presence of zinc. MALDI data also showed that the interaction of \u03b1\u2010crystallin with Zn<jats:sup>+2<\/jats:sup> is quite different from other bivalent metal ions. Bound Zn<jats:sup>+2<\/jats:sup> could be easily removed by dialysis of the complex. The relevance of such weak interaction on the stability of the oligomeric structure of \u03b1\u2010crystallin and its function in the eye lens has been discussed. \u00a9 2010 Wiley Periodicals, Inc. Biopolymers 95: 105\u2013116, 2011.<\/jats:p>","DOI":"10.1002\/bip.21540","type":"journal-article","created":{"date-parts":[[2010,9,20]],"date-time":"2010-09-20T18:41:02Z","timestamp":1285008062000},"page":"105-116","source":"Crossref","is-referenced-by-count":16,"title":["Stabilization of oligomeric structure of \u03b1\u2010crystallin by Zn<sup>+2<\/sup> through intersubunit bridging"],"prefix":"10.1002","volume":"95","author":[{"given":"Srabani","family":"Karmakar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. P.","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,9,20]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.66.1.64-93.2002"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.21.10449"},{"key":"e_1_2_6_4_2","first-page":"10","volume":"34","author":"Horwitz J.","year":"1993","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)53882-5"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.34.19888"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00002a015"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)00775-5"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3110367"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.18.10449"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.50.31973"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404444200"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10930-004-7880-4"},{"key":"e_1_2_6_14_2","first-page":"94","volume-title":"Biochemistry of the Eye","author":"Harding J. J."},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.16.9415"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)50096-X"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90173-6"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(94)90023-X"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)01175-3"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711660110"},{"key":"e_1_2_6_21_2","first-page":"1","volume":"18","author":"Biswas A.","year":"2002","journal-title":"J Surface Sci Technol"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.22.15505"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/381571a0"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2002.03049.x"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M010856200"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.20230"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/bip.20716"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi7011965"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-1529"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(98)00116-0"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.48.34067"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(98)90032-5"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.47.29511"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/bip.20630"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2005.06.009"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-3768.1992.tb04898.x"},{"key":"e_1_2_6_37_2","first-page":"459","volume":"24","author":"Rasi V.","year":"1992","journal-title":"Ann Ophthalmol"},{"key":"e_1_2_6_38_2","first-page":"2050","volume":"15","author":"Singh D.","year":"2009","journal-title":"Mol Vis"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-005-0009-5"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm618"},{"key":"e_1_2_6_41_2","first-page":"125","volume":"11","author":"Nakata K.","year":"1995","journal-title":"Comput Appl Biosci"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.21135"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/6.1.29"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.2.1035"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M407236200"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2005.12.019"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2004.08.010"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi8014967"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.380"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80571-1"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02468"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301991200"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1080\/07315724.2001.10719022"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1159\/000309259"},{"key":"e_1_2_6_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/0048-9697(95)05006-X"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1444-0938.2008.00255.x"},{"key":"e_1_2_6_57_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/115.5.553"},{"key":"e_1_2_6_58_2","first-page":"361","volume":"72","author":"Barash H.","year":"1982","journal-title":"Cornell Vet"}],"container-title":["Biopolymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbip.21540","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bip.21540","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T07:09:51Z","timestamp":1696921791000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bip.21540"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9,20]]},"references-count":57,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2011,2]]}},"alternative-id":["10.1002\/bip.21540"],"URL":"https:\/\/doi.org\/10.1002\/bip.21540","archive":["Portico"],"relation":{},"ISSN":["0006-3525","1097-0282"],"issn-type":[{"value":"0006-3525","type":"print"},{"value":"1097-0282","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9,20]]}}}