{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:18:23Z","timestamp":1790270303485,"version":"4.1.0"},"reference-count":85,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,11,25]],"date-time":"2013-11-25T00:00:00Z","timestamp":1385337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biopolymers"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Cysteine is a uniquely reactive amino acid, capable of undergoing both nucleophlilic and oxidative post\u2010translational modifications. One such oxidation reaction involves the covalent modification of cysteine via the gaseous second messenger nitric oxide (NO), termed <jats:italic>S<\/jats:italic>\u2010nitrosylation (SNO). This dynamic post\u2010translational modification is involved in the redox regulation of proteins across all phylogenic kingdoms. In mammals, calcium\u2010dependent activation of NO synthase triggers the local release of NO, which activates nearby guanylyl cyclases and cGMP\u2010dependent pathways. In parallel, diffusible NO can locally modify redox active cellular thiols, functionally modulating many redox sensitive enzymes. Aberrant SNO is implicated in the pathology of many diseases, including neurodegeneration, inflammation, and stroke. In this review, we discuss current methods to label sites of SNO for biochemical analysis. The most popular method involves a series of biochemical steps to mask free thiols followed by selective nitrosothiol reduction and capture. Other emerging methods include mechanism\u2010based phosphine probes and mercury enrichment chemistry. By bridging new enrichment approaches with high\u2010resolution mass spectrometry, large\u2010scale analysis of protein nitrosylation has highlighted new pathways of oxidative regulation. \u00a9 2013 Wiley Periodicals, Inc. Biopolymers 101: 173\u2013179, 2014.<\/jats:p>","DOI":"10.1002\/bip.22342","type":"journal-article","created":{"date-parts":[[2013,7,5]],"date-time":"2013-07-05T08:44:33Z","timestamp":1373013873000},"page":"173-179","source":"Crossref","is-referenced-by-count":12,"title":["Strategies for profiling native <i>S<\/i>\u2010nitrosylation"],"prefix":"10.1002","volume":"101","author":[{"given":"Jaimeen D.","family":"Majmudar","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Michigan, 930 N. University Ave. Ann Arbor MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brent R.","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Michigan, 930 N. University Ave. Ann Arbor MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,11,25]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"21","volume":"21","author":"Cannan R. K.","year":"1927","journal-title":"Biochem J"},{"key":"e_1_2_6_3_1","first-page":"287","volume":"287","author":"Lim J. C.","year":"2012","journal-title":"J Biol Chem"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M608844200"},{"key":"e_1_2_6_5_1","first-page":"45","volume":"45","author":"Jao S.-C.","year":"2006","journal-title":"J Biochem"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(90)91120-H"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1569"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/35055104"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.2.682"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)80902-U"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.7.2811"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.207381"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2010.10.012"},{"key":"e_1_2_6_14_1","first-page":"28","volume":"28","author":"Lee T.-Y.","year":"2012","journal-title":"Bioinformatics"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.4744"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M111.013441"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi200008b"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M300203200"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.5.C1424"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.45.120403.095959"},{"key":"e_1_2_6_21_1","first-page":"266","volume":"266","author":"Radi R.","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_6_22_1","volume-title":"Free Radicals in Biology and Medicine","author":"Halliwell B.","year":"2007"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.22.8664"},{"key":"e_1_2_6_24_1","first-page":"333","volume":"333","author":"Palmer R. M. J.","year":"1988","journal-title":"Nature"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00424a003"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(89)80219-0"},{"key":"e_1_2_6_27_1","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/S0021-9258(18)54352-0","volume":"266","author":"Leone A. M.","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_6_28_1","first-page":"27","volume":"27","author":"Padgett C. M.","year":"1995","journal-title":"Cell Biochem Biophys"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo00340a038"},{"key":"e_1_2_6_30_1","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1074\/jbc.270.47.28158","volume":"270","author":"Kharitonov V. G.","year":"1995","journal-title":"J Biol Chem"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1281928"},{"key":"e_1_2_6_32_1","first-page":"97","volume":"97","author":"Logager T.","year":"1993","journal-title":"J Phys Chem"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(82)90302-6"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.40.28128"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1006\/niox.1997.0122"},{"key":"e_1_2_6_36_1","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1074\/jbc.270.49.29244","volume":"270","author":"Boese M.","year":"1995","journal-title":"J Biol Chem"},{"key":"e_1_2_6_37_1","first-page":"32","volume":"32","author":"Scott M. J.","year":"1993","journal-title":"Angew Chem Int Ed Engl"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja060186n"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja106290p"},{"key":"e_1_2_6_40_1","first-page":"A1120","volume":"7","author":"Gundimeda U.","year":"1993","journal-title":"FASEB J"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.94.1.94"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(01)76013-4"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(01)00166-2"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2011.06.001"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.4703"},{"key":"e_1_2_6_46_1","first-page":"257","volume":"257","author":"Hwang C.","year":"1992","journal-title":"Science"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11084-011-9243-4"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1999.4084"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.07.002"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx700010h"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2008.2285"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-009-9938-3"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1216623110"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1326-5-49"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.269.3.C739"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2114"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1268"},{"key":"e_1_2_6_58_1","first-page":"269","volume":"269","author":"Hattori Y.","year":"1994","journal-title":"J Biol Chem"},{"key":"e_1_2_6_59_1","first-page":"279","volume":"279","author":"Hao G.","year":"2004","journal-title":"J Biol Chem"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2011.05.033"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2225"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb300607e"},{"key":"e_1_2_6_63_1","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/0092-8674(94)90445-6","volume":"77","author":"Wedegaertner P. B.","year":"1994","journal-title":"Cell"},{"key":"e_1_2_6_64_1","first-page":"301","volume":"301","author":"Wink D. A.","year":"1999","journal-title":"Nitric Oxide Part C"},{"key":"e_1_2_6_65_1","first-page":"1","volume":"2001","author":"SR J.","year":"2001","journal-title":"Sci Signal"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1039\/b901046g"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1039\/b004028m"},{"key":"e_1_2_6_68_1","first-page":"103","volume":"103","author":"Hao G.","year":"2006","journal-title":"Proc Natl Acad Sci U S Am"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.232173"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1545"},{"key":"e_1_2_6_71_1","first-page":"32","volume":"32","author":"Williams D. L. H","year":"1999","journal-title":"Acc Chem Res"},{"key":"e_1_2_6_72_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3765(20020118)8:2<380::AID-CHEM380>3.0.CO;2-P"},{"key":"e_1_2_6_73_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M609684200"},{"key":"e_1_2_6_74_1","first-page":"118","volume":"118","author":"Goldstein S.","year":"1996","journal-title":"J Am Chem Soc"},{"key":"e_1_2_6_75_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00154a007"},{"key":"e_1_2_6_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(95)00081-8"},{"key":"e_1_2_6_77_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00350.2003"},{"key":"e_1_2_6_78_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.4543"},{"key":"e_1_2_6_79_1","first-page":"93","volume":"93","author":"Singh S. P.","year":"1996","journal-title":"Proc Natl Acad Sci U S A"},{"key":"e_1_2_6_80_1","first-page":"26","volume":"26","author":"Li S.","year":"2012","journal-title":"Nitric Oxide"},{"key":"e_1_2_6_81_1","first-page":"11","volume":"11","author":"Zhang J. M.","year":"2009","journal-title":"Org Lett"},{"key":"e_1_2_6_82_1","first-page":"131","volume":"131","author":"Zhang J. M.","year":"2009","journal-title":"J Am Chem Soc"},{"key":"e_1_2_6_83_1","first-page":"266","volume":"266","author":"Seneviratne U. I.","year":"2013","journal-title":"J Am Chem Soc"},{"key":"e_1_2_6_84_1","first-page":"131","volume":"131","author":"Wang H.","year":"2009","journal-title":"J Am Chem Soc"},{"key":"e_1_2_6_85_1","author":"Doulias P.-T.","year":"2013","journal-title":"Sci Signaling"},{"key":"e_1_2_6_86_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1008036107"}],"container-title":["Biopolymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbip.22342","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bip.22342","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T06:02:19Z","timestamp":1694584939000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bip.22342"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,25]]},"references-count":85,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,2]]}},"alternative-id":["10.1002\/bip.22342"],"URL":"https:\/\/doi.org\/10.1002\/bip.22342","archive":["Portico"],"relation":{},"ISSN":["0006-3525","1097-0282"],"issn-type":[{"value":"0006-3525","type":"print"},{"value":"1097-0282","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,25]]}}}