{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:06:32Z","timestamp":1740103592517,"version":"3.37.3"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,5,23]],"date-time":"2015-05-23T00:00:00Z","timestamp":1432339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005010","name":"AIRC","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biopolymers"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Calicheamicin \u03b3<jats:sub>1<\/jats:sub><jats:sup>I<\/jats:sup> (Cal) is a unique molecule in which a DNA binding motif (aryl\u2010tetrasaccharide) is linked to a DNA cleaving moiety (calicheamicinone). The hallmark of this natural product rests in the impressive optimization of these two mechanisms leading to a drug that is extremely efficient in cleaving DNA at well\u2010defined sites. However, the relative contributions of these two structurally distinct domains to the overall process have not been fully elucidated yet. Here, we used different experimental approaches to better dissect the role of the aryl\u2010tetrasaccharide and the enediyne moieties in the DNA sequence selective binding step as well as the in the cleavage reaction. Our results highlight the remarkable cooperation of the two components in producing an amazing molecular machine. The herein presented molecular details of this concerted mechanism of action can be further applied to rationally design more druggable compounds. \u00a9 2014 Wiley Periodicals, Inc. Biopolymers 103: 449\u2013459, 2015.<\/jats:p>","DOI":"10.1002\/bip.22591","type":"journal-article","created":{"date-parts":[[2014,11,20]],"date-time":"2014-11-20T08:57:18Z","timestamp":1416473838000},"page":"449-459","source":"Crossref","is-referenced-by-count":4,"title":["Novel insights on the <scp>DNA<\/scp> interaction of calicheamicin  \u03b3<sub>1<\/sub><sup>I<\/sup>"],"prefix":"10.1002","volume":"103","author":[{"given":"Claudia","family":"Sissi","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences University of Padova Via Marzolo 5 35131 Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Moro","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences University of Padova Via Marzolo 5 35131 Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald M.","family":"Crothers","sequence":"additional","affiliation":[{"name":"Chemistry Department Yale University 225 Prospect Street New Haven CT 06511"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,5,23]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_5_2_1","DOI":"10.1021\/ja00245a051"},{"doi-asserted-by":"publisher","key":"e_1_2_5_3_1","DOI":"10.1021\/ja00245a050"},{"doi-asserted-by":"publisher","key":"e_1_2_5_4_1","DOI":"10.2741\/4181"},{"key":"e_1_2_5_5_1","first-page":"3336","volume":"53","author":"Hinman L. M.","year":"1993","journal-title":"J. Cancer Res"},{"doi-asserted-by":"publisher","key":"e_1_2_5_6_1","DOI":"10.1038\/sj.leu.2403205"},{"doi-asserted-by":"publisher","key":"e_1_2_5_7_1","DOI":"10.1021\/bc010021y"},{"doi-asserted-by":"publisher","key":"e_1_2_5_8_1","DOI":"10.1371\/journal.pone.0053518"},{"key":"e_1_2_5_9_1","first-page":"1490","volume":"7","author":"Bross P. F.","year":"2001","journal-title":"Clin Cancer Res"},{"doi-asserted-by":"publisher","key":"e_1_2_5_10_1","DOI":"10.1586\/17474086.2014.924849"},{"doi-asserted-by":"publisher","key":"e_1_2_5_11_1","DOI":"10.1021\/ja00029a030"},{"doi-asserted-by":"publisher","key":"e_1_2_5_12_1","DOI":"10.1126\/science.2717946"},{"doi-asserted-by":"publisher","key":"e_1_2_5_13_1","DOI":"10.1126\/science.3240341"},{"doi-asserted-by":"publisher","key":"e_1_2_5_14_1","DOI":"10.1021\/ja00182a053"},{"doi-asserted-by":"publisher","key":"e_1_2_5_15_1","DOI":"10.1021\/ja00049a069"},{"doi-asserted-by":"publisher","key":"e_1_2_5_16_1","DOI":"10.1002\/tcr.201200005"},{"doi-asserted-by":"publisher","key":"e_1_2_5_17_1","DOI":"10.1002\/anie.199408581"},{"doi-asserted-by":"publisher","key":"e_1_2_5_18_1","DOI":"10.1021\/ja00051a063"},{"doi-asserted-by":"publisher","key":"e_1_2_5_19_1","DOI":"10.1002\/chir.20990"},{"doi-asserted-by":"publisher","key":"e_1_2_5_20_1","DOI":"10.1006\/jmbi.1996.0718"},{"doi-asserted-by":"publisher","key":"e_1_2_5_21_1","DOI":"10.1021\/bi00168a029"},{"doi-asserted-by":"publisher","key":"e_1_2_5_22_1","DOI":"10.1021\/ja00088a005"},{"doi-asserted-by":"publisher","key":"e_1_2_5_23_1","DOI":"10.1073\/pnas.88.17.7464"},{"doi-asserted-by":"publisher","key":"e_1_2_5_24_1","DOI":"10.1021\/ja00088a006"},{"doi-asserted-by":"publisher","key":"e_1_2_5_25_1","DOI":"10.1021\/bi992227g"},{"doi-asserted-by":"publisher","key":"e_1_2_5_26_1","DOI":"10.1073\/pnas.96.19.10643"},{"doi-asserted-by":"publisher","key":"e_1_2_5_27_1","DOI":"10.1021\/bc900128h"},{"key":"e_1_2_5_28_1","first-page":"2925","volume":"58","author":"Lode H. N.","year":"1998","journal-title":"Cancer Res"},{"doi-asserted-by":"publisher","key":"e_1_2_5_29_1","DOI":"10.1021\/ja00126a014"},{"unstructured":".MOE (Molecular Operating Environment). Chemical Computing Group Inc: 1010 Sherbooke St West Suite#910 Montreal Quebec Canada 2012 version 2013.08;.","key":"e_1_2_5_30_1"},{"doi-asserted-by":"publisher","key":"e_1_2_5_31_1","DOI":"10.1002\/chem.200700504"},{"doi-asserted-by":"publisher","key":"e_1_2_5_32_1","DOI":"10.1021\/ja00136a004"},{"doi-asserted-by":"publisher","key":"e_1_2_5_33_1","DOI":"10.1016\/S0040-4020(01)80622-0"},{"doi-asserted-by":"publisher","key":"e_1_2_5_34_1","DOI":"10.1016\/j.abb.2006.03.027"},{"doi-asserted-by":"publisher","key":"e_1_2_5_35_1","DOI":"10.1529\/biophysj.106.097451"},{"doi-asserted-by":"publisher","key":"e_1_2_5_36_1","DOI":"10.1093\/nar\/gkr699"},{"doi-asserted-by":"publisher","key":"e_1_2_5_37_1","DOI":"10.1021\/ja00017a038"},{"doi-asserted-by":"publisher","key":"e_1_2_5_38_1","DOI":"10.1021\/bi00003a035"},{"doi-asserted-by":"publisher","key":"e_1_2_5_39_1","DOI":"10.1002\/anie.199401831"},{"doi-asserted-by":"publisher","key":"e_1_2_5_40_1","DOI":"10.1021\/ol0494919"},{"doi-asserted-by":"publisher","key":"e_1_2_5_41_1","DOI":"10.1002\/anie.199408551"},{"doi-asserted-by":"publisher","key":"e_1_2_5_42_1","DOI":"10.1093\/nar\/4.10.3573"},{"doi-asserted-by":"publisher","key":"e_1_2_5_43_1","DOI":"10.1021\/bi00065a013"}],"container-title":["Biopolymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbip.22591","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bip.22591","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T23:22:34Z","timestamp":1694560954000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bip.22591"}},"subtitle":[],"editor":[{"given":"Stephen","family":"Levene","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,5,23]]},"references-count":42,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1002\/bip.22591"],"URL":"https:\/\/doi.org\/10.1002\/bip.22591","archive":["Portico"],"relation":{},"ISSN":["0006-3525","1097-0282"],"issn-type":[{"type":"print","value":"0006-3525"},{"type":"electronic","value":"1097-0282"}],"subject":[],"published":{"date-parts":[[2015,5,23]]}}}