{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T15:37:20Z","timestamp":1786808240324,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,12,22]],"date-time":"2015-12-22T00:00:00Z","timestamp":1450742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biopolymers"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Scaffold\u2010based tissue engineering is considered as a promising approach in the regenerative medicine. Graft instability of collagen, by causing poor mechanical properties and rapid degradation, and their hard handling remains major challenges to be addressed. In this research, a composite structured nano\u2010\/microfibrous scaffold, made from a mixture of chitosan\u2013\u00df\u2010glycerol phosphate\u2013gelatin (chitosan\u2013GP\u2013gelatin) using a standard electrospinning set\u2010up was developed. Gelatin\u2013acid acetic and chitosan \u00df\u2010glycerol phosphate\u2013HCL solutions were prepared at ratios of 30\/70, 50\/50, 70\/30 (w\/w) and their mechanical and biological properties were engineered. Furthermore, the pore structure of the fabricated nanofibrous scaffolds was investigated and predicted using a theoretical model. Higher gelatin concentrations in the polymer blend resulted in significant increase in mean pore size and its distribution. Interaction between the scaffold and the contained cells was also monitored and compared in the test and control groups. Scaffolds with higher chitosan concentrations showed higher rate of cell attachment with better proliferation property, compared with gelatin\u2010only scaffolds. The fabricated scaffolds, unlike many other natural polymers, also exhibit non\u2010toxic and biodegradable properties in the grafted tissues. In conclusion, the data clearly showed that the fabricated biomaterial is a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell\u2010based tissue engineering, especially in wound healing practices. These results suggested the potential of using mesoporous composite chitosan\u2013GP\u2013gelatin fibrous scaffolds for engineering three\u2010dimensional tissues with different inherent cell characteristics. \u00a9 2015 Wiley Periodicals, Inc. Biopolymers 105: 163\u2013175, 2016.<\/jats:p>","DOI":"10.1002\/bip.22764","type":"journal-article","created":{"date-parts":[[2015,11,13]],"date-time":"2015-11-13T16:00:50Z","timestamp":1447430450000},"page":"163-175","source":"Crossref","is-referenced-by-count":18,"title":["Hybrid chitosan\u2013\u00df\u2010glycerol phosphate\u2013gelatin nano\u2010\/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering"],"prefix":"10.1002","volume":"105","author":[{"given":"Marzieh","family":"Lotfi","sequence":"first","affiliation":[{"name":"Department of Modern Sciences &amp; Technologies School of Medicine Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roohollah","family":"Bagherzadeh","sequence":"additional","affiliation":[{"name":"Advanced Textile Materials and Technology Research Institute (ATMT), Textile Engineering Department Amirkabir University of Technology Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hojjat","family":"Naderi\u2010Meshkin","sequence":"additional","affiliation":[{"name":"Stem Cell and Regenerative Medicine Research Group Iranian Academic Center for Education, Culture and Research (ACECR) Khorasan Razavi Branch Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elahe","family":"Mahdipour","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, School of Medicine Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asghar","family":"Mafinezhad","sequence":"additional","affiliation":[{"name":"Pathology Department of Shahid Kamyab (Emdadi) Hospital Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid Reza","family":"Sadeghnia","sequence":"additional","affiliation":[{"name":"Neurocognitive Research Center, School of Medicine Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habibollah","family":"Esmaily","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Epidemiology School of Health, Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masoud","family":"Maleki","sequence":"additional","affiliation":[{"name":"Cutaneous Leishmaniasis Research Center School of Medicine, Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Halimeh","family":"Hasssanzadeh","sequence":"additional","affiliation":[{"name":"Stem Cell and Regenerative Medicine Research Group Iranian Academic Center for Education, Culture and Research (ACECR) Khorasan Razavi Branch Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majid","family":"Ghayaour\u2010Mobarhan","sequence":"additional","affiliation":[{"name":"Biochemistry of Nutrition Research Center, School of Medicine Mashhad University of Medicine Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid Reza","family":"Bidkhori","sequence":"additional","affiliation":[{"name":"Stem Cell and Regenerative Medicine Research Group Iranian Academic Center for Education, Culture and Research (ACECR) Khorasan Razavi Branch Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad Reza","family":"Bahrami","sequence":"additional","affiliation":[{"name":"Stem Cell and Regenerative Medicine Research Group Iranian Academic Center for Education, Culture and Research (ACECR) Khorasan Razavi Branch Mashhad Iran"},{"name":"Cell and Molecular Biotechnology Research Group Institute of Biotechnology, Ferdowsi University of Mashhad Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,12,22]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm200680q"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1055"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2009.07.024"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1069210"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.08.004"},{"key":"e_1_2_5_7_1","volume-title":"Handbook of Nanostructured Biomaterials and Their Applications in Nanobiotechnology","author":"Nalwa H. 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