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Here, we report that the peptide segment corresponding to AdVpVI (positions 33\u201355) can induce positive membrane curvature together with membrane perturbation. The enhanced perturbation ability of the peptide was observed for membranes containing negatively charged phospholipids. Based on the liposome leakage assay, substitution of leucine at position 40 to other aliphatic (isoleucine) and aromatic (phenylalanine and tryptophan) residues yielded a similar degree of membrane perturbation by the peptides, which was considerably diminished by the substitution to glutamine. Further studies using the wild\u2010type AdVpVI (33\u201355) (WT) and phenylalanine\u2010substituted peptides (L40F) demonstrated that both peptides have positive membrane\u2010curvature\u2010inducing ability. These peptides showed higher binding affinity to 50\u2010nm large unilamellar vesicles (LUVs) than to 200\u2010nm LUVs. However, no enhanced perturbation by these peptides was observed for 50\u2010nm LUVs compared to 200\u2010nm LUVs, suggesting that both the original membrane curvature and the additional strain due to peptide insertion affect the membrane perturbation ability of these peptides. In the case of L40F, this peptide rather had a lower membrane perturbation ability for 50\u2010nm LUVs than for 200\u2010nm LUVs, which can be attributed to possible shallower binding of L40F on membranes. \u00a9 2016 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 106: 430\u2013439, 2016.<\/jats:p>","DOI":"10.1002\/bip.22797","type":"journal-article","created":{"date-parts":[[2016,6,6]],"date-time":"2016-06-06T13:54:36Z","timestamp":1465221276000},"page":"430-439","source":"Crossref","is-referenced-by-count":7,"title":["Effect of amino acid substitution in the hydrophobic face of amphiphilic peptides on membrane curvature and perturbation: N\u2010terminal helix derived from adenovirus internal protein VI as a model"],"prefix":"10.1002","volume":"106","author":[{"given":"Tomo","family":"Murayama","sequence":"first","affiliation":[{"name":"Institute for Chemical Research Kyoto University Uji Kyoto 611\u20100011 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00edlvia","family":"Pujals","sequence":"additional","affiliation":[{"name":"Institute for Chemical Research Kyoto University Uji Kyoto 611\u20100011 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisaaki","family":"Hirose","sequence":"additional","affiliation":[{"name":"Institute for Chemical Research Kyoto University Uji Kyoto 611\u20100011 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ikuhiko","family":"Nakase","sequence":"additional","affiliation":[{"name":"Nanoscience and Nanotechnology Research Center, Research Organization for the 21st Century Osaka Prefecture University Naka\u2010Ku, Sakai Osaka 599\u20108570 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiroh","family":"Futaki","sequence":"additional","affiliation":[{"name":"Institute for Chemical Research Kyoto University Uji Kyoto 611\u20100011 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,7,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920312804142138"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.10.022"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0282(2000)55:1<4::AID-BIP30>3.0.CO;2-M"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2008.09.013"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114454"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00116-13"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.08.033"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.03.043"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/29555"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb4002987"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200107075"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600714"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02321-10"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3381086"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3011.1990.tb00939.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708354105"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb1194"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.107.109967"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.360330713"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.4.1992-2000.2005"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(95)46006-3"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btn392"},{"key":"e_1_2_6_24_1","first-page":"369","volume":"18","author":"Fauch\u00e8re J. 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