{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T19:38:21Z","timestamp":1790365101151,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,9,25]],"date-time":"2017-09-25T00:00:00Z","timestamp":1506297600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004584","name":"Conseil R\u00e9gional de Bretagne","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004584","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biopolymers"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Increasing bacterial resistance towards antibiotics has stimulated research for novel antimicrobials. Proteins acting on bacterial membranes could be a solution. Lysozyme has been proven active against <jats:italic>E. coli<\/jats:italic> by disruption of both outer and cytoplasmic membranes, with dry\u2010heating increasing lysozyme activity. Dry\u2010heated lysozyme (DH\u2010L) is a mixture of isoforms (isoaspartyl, native\u2010like and succinimide lysozymes), giving rise to two questions: what effects does each form have, and which physicochemical properties are critical as regards the antibacterial activity? These issues were investigated by fractionating DH\u2010L, analyzing structural properties of each fraction, and testing each fraction in vivo on bacteria and in vitro on membrane models. Positive net charge, hydrophobicity and molecular flexibility of the isoforms seem key parameters for their interaction with <jats:italic>E. coli<\/jats:italic> membranes. The succinimide lysozyme fraction, the most positive, flexible and hydrophobic, shows the highest antimicrobial activity, induces the strongest bacterial membrane disruption and is the most surface active on model lipid monolayers. Moreover, each fraction appears less efficient than DH\u2010L against <jats:italic>E. coli<\/jats:italic>, indicating a synergetic cooperation between lysozyme isoforms. The bacterial membrane modifications induced by one isoform could facilitate the subsequent action of the other isoforms.<\/jats:p>","DOI":"10.1002\/bip.23040","type":"journal-article","created":{"date-parts":[[2017,9,25]],"date-time":"2017-09-25T10:26:22Z","timestamp":1506335182000},"source":"Crossref","is-referenced-by-count":11,"title":["Antimicrobial activity of lysozyme isoforms: Key molecular features"],"prefix":"10.1002","volume":"107","author":[{"given":"Melanie","family":"Derde","sequence":"first","affiliation":[{"name":"STLO, UMR1253, Agrocampus Ouest, INRA Rennes F\u201035 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Vi\u00e9","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Rennes 1, Institut de Physique de Rennes, UMR6251, CNRS Rennes F\u201035 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astrid","family":"Walrant","sequence":"additional","affiliation":[{"name":"Sorbonne 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