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Previous studies in our group have shown that conjugation of amphiphilic tobramycin to other antibacterials enhance their latent outer membrane permeabilizing and efflux blocking activity toward Gram\u2010negative pathogens including <jats:italic>Pseudomonas aeruginosa<\/jats:italic>. Herein, we describe the antimicrobial adjuvant properties of amphiphilic lysine ligated to tobramycin. The most potent amphiphilic lysine\u2010tobramycin conjugate <jats:bold>3<\/jats:bold> potentiated the antibacterial efficacy of 8 clinically used antibiotics against wild type, multidrug\u2010 and extensively drug\u2010resistant <jats:italic>P. aeruginosa<\/jats:italic> isolates from Canadian hospitals whereas amphiphilic lysine <jats:bold>4<\/jats:bold> did not. Antibiotics that are synergistic with conjugate <jats:bold>3<\/jats:bold> included moxifloxacin, ciprofloxacin, erythromycin, chloramphenicol, trimethoprim, novobiocin, linezolid, and fosfomycin. Out of these 8 antibiotics, novobiocin showed highest synergy.<\/jats:p>","DOI":"10.1002\/bip.23091","type":"journal-article","created":{"date-parts":[[2017,12,5]],"date-time":"2017-12-05T11:30:59Z","timestamp":1512473459000},"source":"Crossref","is-referenced-by-count":19,"title":["Amphiphilic lysine conjugated to tobramycin synergizes legacy antibiotics against wild\u2010type and multidrug\u2010resistant <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1002","volume":"111","author":[{"given":"Yinfeng","family":"Lyu","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Manitoba Winnipeg MB R3T 2N2 Canada"},{"name":"Institute of Animal Nutrition, Northeast Agricultural University Harbin Heilongjiang 150030 P.R. 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