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Existing antifungal treatment plans have high mammalian toxicity and increasing drug resistance, demonstrating the dire need for new, nontoxic therapeutics. Antimicrobial peptoids are one alternative to combat this issue. Our lab has recently identified a tripeptoid, AEC5, with promising efficacy and selectivity against <jats:italic>C. neoformans<\/jats:italic>. Here, we report studies into the broad\u2010spectrum efficacy, killing kinetics, mechanism of action, <jats:italic>in vivo<\/jats:italic> half\u2010life, and subchronic toxicity of this compound. Most notably, these studies have demonstrated that AEC5 rapidly reduces fungal burden, killing all viable fungi within 3 hours. Additionally, AEC5 has an <jats:italic>in vivo<\/jats:italic> half\u2010life of 20+ hours and no observable <jats:italic>in vivo<\/jats:italic> toxicity following 28\u2009days of daily injections. This research represents an important step in the characterization of AEC5 as a practical treatment option against <jats:italic>C. neoformans<\/jats:italic> infections.<\/jats:p>","DOI":"10.1002\/bip.23276","type":"journal-article","created":{"date-parts":[[2019,4,2]],"date-time":"2019-04-02T17:20:13Z","timestamp":1554225613000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Toward a clinical antifungal peptoid: Investigations into the therapeutic potential of AEC5"],"prefix":"10.1002","volume":"110","author":[{"given":"Sabrina K.","family":"Spicer","sequence":"first","affiliation":[{"name":"Department of Chemistry Middle Tennessee State University  Murfreesboro Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aarthi","family":"Subramani","sequence":"additional","affiliation":[{"name":"Department of Biology Middle Tennessee State University  Murfreesboro Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelica L.","family":"Aguila","sequence":"additional","affiliation":[{"name":"Department of Chemistry Middle Tennessee State University  Murfreesboro Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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