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The clam inhabits a sulfur\u2010rich environment and HbIII is the only hemoprotein present in the system which does not yet have a structure described elsewhere. It is known that HbIII exists as a heterodimer with hemoglobin II (HbII) to generate the stable Oxy(HbII\u2010HbIII) complex but it remains unknown if HbIII can form a homodimeric species. Here, a new chromatographic methodology to separate OxyHbIII from the HbII\u2010HbIII dimer has been developed, employing a fast performance liquid chromatography and ionic exchange chromatography column. The nature of OxyHbIII in solution at concentrations from 1.6\u00a0mg\/mL to 20.4\u00a0mg\/mL was studied using small angle X\u2010ray scattering (SAXS). The results show that at all concentrations, the Oxy(HbIII\u2010HbIII) dimer dominates in solution. However, as the concentration increases to nonphysiological values, 20.4\u00a0mg\/mL, HbIII forms a 30% tetrameric fraction. Thus, there is a direct relationship between the Oxy(HbIII\u2010HbIII) oligomeric form and hemoglobin concentration. We suggest it is likely that the OxyHbIII dimer contributes to active oxygen transport in tissues of <jats:italic>L pectinata<\/jats:italic>, where the Oxy(HbII\u2010HbIII) complex is not present.<\/jats:p>","DOI":"10.1002\/bip.23427","type":"journal-article","created":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T08:01:27Z","timestamp":1617264087000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>SAXS<\/scp> structure of homodimeric <scp>oxyHemoglobin III<\/scp> from bivalve <scp><i>Lucina pectinata<\/i><\/scp>"],"prefix":"10.1002","volume":"112","author":[{"given":"Darya","family":"Marchany\u2010Rivera","sequence":"first","affiliation":[{"name":"Chemistry Department and Industrial Biotechnology Program University of Puerto Rico  Mayag\u00fcez Puerto Rico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael A.","family":"Estremera\u2010And\u00fajar","sequence":"additional","affiliation":[{"name":"Natural Sciences Department University of Puerto Rico  Aguadilla Puerto Rico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Nieves\u2010Marrero","sequence":"additional","affiliation":[{"name":"Natural Sciences Department University of Puerto Rico  Aguadilla Puerto Rico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos R.","family":"Ruiz\u2010Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Natural Sciences Department University of Puerto Rico  Aguadilla Puerto Rico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Bauer","sequence":"additional","affiliation":[{"name":"Hauptman\u2010Woodward Medical Research Institute  Buffalo New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5506-3434","authenticated-orcid":false,"given":"Juan","family":"L\u00f3pez\u2010Garriga","sequence":"additional","affiliation":[{"name":"Chemistry Department and Industrial Biotechnology Program University of Puerto Rico  Mayag\u00fcez Puerto Rico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1144\/GSL.SP.2000.177.01.12"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0010-406X(65)90342-7"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2307\/1539582"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)46185-0"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1007\/BF00353264","volume":"125","author":"Frenkiel L.","year":"1996","journal-title":"Mar. 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