{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T04:36:38Z","timestamp":1783658198968,"version":"3.55.0"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T00:00:00Z","timestamp":1637625600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000070","name":"National Institute of Biomedical Imaging and Bioengineering","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000070","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006084","name":"Office of AIDS Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006084","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biopolymers"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Routine patient testing for viral infections is critical to identify infected individuals for treatment and to prevent spreading of infections to others. Developing robust and reliable diagnostic tools to detect nucleic acids of viruses at the point\u2010of\u2010care could greatly assist the clinical management of viral infections. The remarkable stability and high binding affinity of peptide nucleic acids (PNAs) to target nucleic acids could make PNA\u2010based biosensors an excellent starting point to develop new nucleic acid detection technologies. We report the application of cyclopentane\u2010modified PNAs to capture target nucleic acids in a microfluidic channel, and the use of bioorthogonal PNAs conjugated to gold nanoparticles as probes to semi\u2010quantitatively signal the presence of a target nucleic acid derived from HIV\u20101. The basic results presented could be used to develop more advanced devices to detect nucleic acids from viruses such as HIV, SARS\u2010CoV\u20102, and a wide range of other human diseases.<\/jats:p>","DOI":"10.1002\/bip.23481","type":"journal-article","created":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T12:51:33Z","timestamp":1637671893000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Cyclopentane peptide <scp>nucleic acid<\/scp>: Gold nanoparticle conjugates for the detection of nucleic acids in a microfluidic format"],"prefix":"10.1002","volume":"113","author":[{"given":"Harsha","family":"Amarasekara","sequence":"first","affiliation":[{"name":"Synthetic Bioactive Molecules Section, Laboratory of Bioorganic Chemistry (LBC) National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaylyn M.","family":"Oshaben","sequence":"additional","affiliation":[{"name":"Synthetic Bioactive Molecules Section, Laboratory of Bioorganic Chemistry (LBC) National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health  Bethesda Maryland USA"},{"name":"Department of Research and Development Altratech Ltd.  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kendra B.","family":"Jeans","sequence":"additional","affiliation":[{"name":"Synthetic Bioactive Molecules Section, Laboratory of Bioorganic Chemistry (LBC) National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paniz","family":"Rezvan Sangsari","sequence":"additional","affiliation":[{"name":"Biomedical Engineering and Physical Science Shared Resource National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole Y.","family":"Morgan","sequence":"additional","affiliation":[{"name":"Biomedical Engineering and Physical Science Shared Resource National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"O'Farrell","sequence":"additional","affiliation":[{"name":"Department of Research and Development Altratech Ltd.  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7195-7764","authenticated-orcid":false,"given":"Daniel H.","family":"Appella","sequence":"additional","affiliation":[{"name":"Synthetic Bioactive Molecules Section, Laboratory of Bioorganic Chemistry (LBC) National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.0c02624"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-008-0079-7"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.aids.0000279710.47298.5c"},{"key":"e_1_2_8_5_1","volume":"31","author":"Basiri A.","year":"2020","journal-title":"Rev. Med. Virol."},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspa.2020.0398"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/650389"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1962210"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-1669(99)80013-5"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-1669(00)00170-1"},{"key":"e_1_2_8_11_1","first-page":"583","volume":"90","author":"Quijano E.","year":"2017","journal-title":"Yale J. Biol. Med."},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja046280q"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/ol0480980"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/ol0348811"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1249"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/382607a0"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201209684"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja046785g"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac900525k"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10404-010-0638-8"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(92)80293-4"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05058"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200210)23:20<3461::AID-ELPS3461>3.0.CO;2-8"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.1387591"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.4816934"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.orglett.8b03374"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2005.06.063"}],"container-title":["Biopolymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bip.23481","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bip.23481","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bip.23481","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T19:10:34Z","timestamp":1692990634000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bip.23481"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,23]]},"references-count":26,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["10.1002\/bip.23481"],"URL":"https:\/\/doi.org\/10.1002\/bip.23481","archive":["Portico"],"relation":{},"ISSN":["0006-3525","1097-0282"],"issn-type":[{"value":"0006-3525","type":"print"},{"value":"1097-0282","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,23]]},"assertion":[{"value":"2021-07-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e23481"}}