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Among microbial infections, fungal diseases remain overlooked, with limited therapeutic options and high recurrence. Cutaneous cryptococcosis is an opportunistic fungal infection triggered by mechanical inoculation or hematogenous dissemination of the yeast that causes cryptococcal pneumonia and meningitis. Every year, <jats:italic>Cryptococcus neoformans<\/jats:italic> endanger the lives of immunosuppressed hosts, resulting in 180,000 deaths per year. Nonetheless, healthy individuals can also be affected by this fungal infection. Cryptococcosis has a restricted and expensive therapeutic regimen with no topical approach to skin manifestations. This study sought to create a 3D printable biodegradable polymeric hydrogel carrying ketoconazole, a low\u2010cost antifungal drug with reported anticryptococcal activity. The developed hydrogel exhibited good 3D printability and rheological properties, including a pseudoplastic behavior. The FTIR spectra of cross\u2010linked hydrogels revealed interactions between alginate and Ca<jats:sup>+2<\/jats:sup>, referred to as the egg\u2010box model, indicated by the decrease in peaks at 1600 and 1410\u2009cm<jats:sup>\u22121<\/jats:sup>. Furthermore, the hydrogel loaded with ketoconazole showed remarkable antifungal activity against <jats:italic>C. neoformans<\/jats:italic> strains indicated by inhibition zones, which cross\u2010linking did not seem to affect its antifungal performance. The developed material remained structurally stable for up to 12\u2009days (288\u2009h) in swelling studies, and preliminary cytotoxicity performed with V79 cells indicates potential for in\u00a0vivo studies and topical application.<\/jats:p>","DOI":"10.1002\/bip.23638","type":"journal-article","created":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T12:28:37Z","timestamp":1730204917000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>3D<\/scp> Printable Alginate\u2013Chitosan Hydrogel Loaded With Ketoconazole Exhibits Anticryptococcal Activity"],"prefix":"10.1002","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9418-4610","authenticated-orcid":false,"given":"Manoela Almeida Martins","family":"Mace","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o Em Ci\u00eancias Farmac\u00eauticas Universidade Federal do Rio Grande do Sul (UFRGS)  Porto Alegre 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