{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T11:03:08Z","timestamp":1783162988597,"version":"3.54.6"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,2,1]],"date-time":"2004-02-01T00:00:00Z","timestamp":1075593600000},"content-version":"vor","delay-in-days":3958,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biopolymers"],"published-print":{"date-parts":[[1993,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The secondary structure transformation of \u03b2\u2010lactoglobulin from a predominantly \u03b2\u2010structure into a predominantly \u03b1\u2010helical one, under the influence of solvent polarity changes is reversible. Independent of the alcohol used \u2014 methanol, ethanol, or 2\u2010propanol \u2014 the midpoints of the observed structural transformation occur around dielectric constant \u03b5 \u2248 60. The structural change destroying the hydrophobic core formed by the \u03b2\u2010barrel structure leads, at room temperature, to the dissociation of the retinol\/\u03b2\u2010lactoglobulin complex in the neighborhood of dielectric constant \u03b5 \u2248 50. However, when the dielectric constant of the medium is raised back to \u03b5 \u2248 70 by the decrease of the temperature, both the refolding of BLG into a \u03b2\u2010structure and the reassociation of the retinol\/\u03b2\u2010lactoglobulin complex are observed. The esterification of \u03b2\u2010lactoglobulin carboxyl groups has two effects: on the one hand it accelerates the \u03b2\u2010strand \u2192 \u03b1\u2010helix transition induced by alcohols. On the other hand, the esterification of \u03b2\u2010lactoglobulin strengthens its interaction with retinol as it may be deduced from the smaller apparent dissociation constant of retinol\/methylated \u03b2\u2010lactoglobulin complex. The binding of retinol to modified or unmodified \u03b2\u2010lactoglobulin has no influence (stabilizing or destabilizing) on the folding changes induced by alcohol. \u00a9 1993 John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/bip.360330408","type":"journal-article","created":{"date-parts":[[2004,12,30]],"date-time":"2004-12-30T03:05:43Z","timestamp":1104375943000},"page":"589-598","source":"Crossref","is-referenced-by-count":61,"title":["Reversible effects of medium dielectric constant on structural transformation of \u03b2\u2010lactoglobulin and its retinol binding"],"prefix":"10.1002","volume":"33","author":[{"given":"Eric","family":"Dufour","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Bertrand\u2010Harb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Haertl\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,2]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(91)90005-G"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(88)90031-X"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)44953-3"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(84)81360-0"},{"key":"e_1_2_1_6_2","first-page":"357","volume":"42","author":"Diaz de Villegas M. 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