{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:56:47Z","timestamp":1790873807975,"version":"4.1.0"},"reference-count":71,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T00:00:00Z","timestamp":1771200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T00:00:00Z","timestamp":1771200000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biopolymers"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study focused on functionalizing chitosan with nicotinic acid, the active form of vitamin B3, to obtain a new derivative (Chi\n                    <jats:sub>Nico<\/jats:sub>\n                    ) with enhanced solubility at physiological pH, improved proton buffering capacity, and in\u00a0vitro anticancer activity, and to develop resveratrol\u2010loaded nanoparticles (nChi\n                    <jats:sub>Nico\u2010RES<\/jats:sub>\n                    ) for enhanced anticancer performance. Chitosan was modified through EDC\u2010mediated amidation, and successful conjugation was confirmed by FTIR,\n                    <jats:sup>1<\/jats:sup>\n                    H NMR, and GPC\/SEC analyses. Nicotinic acid grafting increased molecular weight, introduced characteristic amide signals, improved solubility at physiological pH, and enhanced proton buffering capacity. Nanoparticles were prepared by ionotropic gelation and showed sizes of 100\u2013140\u2009nm, PDI values below 0.4, and a positive surface charge of +18 to +20\u2009mV. Blank nanoparticles exhibited minimal cytotoxicity, while resveratrol\u2010loaded formulations demonstrated significant anticancer activity in HeLa cervical cancer cells and HT\u201029 human colon adenocarcinoma cell line. Notably, nChi\n                    <jats:sub>Nico\u2010RES<\/jats:sub>\n                    reduced HeLa and HT\u201029 cell viability more effectively than free resveratrol and nanoparticles based on unmodified chitosan, indicating an additive contribution from nicotinic acid. In contrast, the cytotoxic effect on healthy BJ fibroblasts remained considerably lower, supporting the biocompatibility and selective potential of the system. Overall, nicotinic acid modification improves chitosan's carrier performance and offers a novel strategy by combining two natural bioactive molecules within a single nanoparticle platform.\n                  <\/jats:p>","DOI":"10.1002\/bip.70085","type":"journal-article","created":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T07:35:44Z","timestamp":1771313744000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Nicotinic Acid\u2013Modified Chitosan Nanoparticles for Enhanced Resveratrol Delivery and Anticancer Activity"],"prefix":"10.1002","volume":"117","author":[{"given":"Sema","family":"\u015eent\u00fcrk","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering Istanbul Health and Technology University, Faculty of Engineering and Natural Sciences  Istanbul T\u00fcrkiye"},{"name":"Department of Chemical Engineering Istanbul University\u2010Cerrahpa\u015fa, Faculty of Engineering  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6zlem","family":"Kaplan","sequence":"additional","affiliation":[{"name":"Department of Genetics and Bioengineering Alanya Alaaddin Keykubat University, Rafet Kay\u0131\u015f Faculty of Engineering  Antalya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevser","family":"Bal","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Istanbul University\u2010Cerrahpa\u015fa, Faculty of Engineering  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sibel","family":"K\u00fc\u00e7\u00fckertu\u011frul \u00c7elik","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Istanbul University\u2010Cerrahpa\u015fa, Faculty of Engineering  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazan","family":"G\u00f6k\u015fen Tosun","sequence":"additional","affiliation":[{"name":"Department of Medical Services and Techniques Tokat Gaziosmanpa\u015fa University, Tokat Vocational School of Health Services  Tokat T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet Koray","family":"G\u00f6k","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Istanbul University\u2010Cerrahpa\u015fa, Faculty of Engineering  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-024-01889-y"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym16060843"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmt.2023.09.001"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/1061186X.2025.2502956"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/targets2040018"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12122056"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2016.00008"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cnr2.1353"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina61060981"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2015.01.003"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2014.11.004"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-021-02099-0"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.24200\/jogcr.11.1.10"},{"issue":"2","key":"e_1_2_10_15_1","first-page":"441","article-title":"Preparation, Characterization, and Cellular Uptake of Resveratrol\u2010Loaded Trimethyl Chitosan Nanoparticles","volume":"27","author":"Min J. 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