{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T13:47:34Z","timestamp":1761486454999,"version":"build-2065373602"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,11,13]],"date-time":"2001-11-13T00:00:00Z","timestamp":1005609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp;amp; Bioengineering"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>As measured by the toluene\u2010induced bioluminescent response of <jats:italic>Pseudomonas putida<\/jats:italic> TVA8 in batch experiments, toluene dioxygenase (Tod) enzyme activities are dependent on toluene concentration between 0 and 30 mg\/L. To provide a measure of the Tod activity for use in Michaelis\u2010Menten competitive\u2010inhibition kinetics, a correlation between toluene concentration and induced Tod activity as measured by an induced bioluminescent response of <jats:italic>P. putida<\/jats:italic> TVA8 is presented as a nondimensional Tod activity parameter. A packed\u2010bed, radial\u2010flow bioreactor (RFB) using the bioreporter <jats:italic>P. putida<\/jats:italic> TVA8A serves as the model system for studying the effect of the enzyme activity parameter on model predictions of vapor\u2010phase toluene oxidation and trichloroethylene (TCE) cometabolism. Mass balances were performed on a differential section of the RFB to describe the radial transport of vapor\u2010phase toluene and TCE through a bulk gas phase and the concomitant biological reaction in a stationary biofilm phase. The finite\u2010element Galerkin weak\u2010statement formulation with first\u2010order basis functions was used to find the optimum solution to the highly nonlinear, coupled equations. For this RFB system with toluene concentrations less than 1 mg\/L in the bulk gas phase, the Tod activity parameter enables accurate predictions of steady\u2010state TCE degradation rate (0.27 \u03bcg TCE\/min). \u00a9 2001 John Wiley &amp; Sons, Inc. Biotechnol Bioeng 76: 341\u2013350, 2001.<\/jats:p>","DOI":"10.1002\/bit.10083","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T17:17:12Z","timestamp":1030295832000},"page":"341-350","source":"Crossref","is-referenced-by-count":8,"title":["Quantification of toluene dioxygenase induction and kinetic modeling of TCE cometabolism by <i>Pseudomonas putida<\/i> TVA8"],"prefix":"10.1002","volume":"76","author":[{"given":"J.T.","family":"Shingleton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.A.","family":"Applegate","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.J.","family":"Baker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.S.","family":"Sayler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.R.","family":"Bienkowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,11,13]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"2730","DOI":"10.1128\/AEM.64.7.2730-2735.1998","article-title":"A chromosomally based tod\u2010luxCDABE whole\u2010cell reporter for benzene, toluene, ethylbenzene, and xylene (BTEX) sensing","volume":"64","author":"Applegate BM","year":"1998","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_1_3_1","unstructured":"1986 McGraw\u2010Hill New York JE Bailey DF Ollis Biochemical engineering fundamentals 928"},{"key":"e_1_2_1_4_1","first-page":"341","volume-title":"Finite elements 123","author":"Baker AJ","year":"1991"},{"key":"e_1_2_1_5_1","first-page":"780","volume-title":"Transport phenomena","author":"Bird RB","year":"1960"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260450509"},{"key":"e_1_2_1_7_1","first-page":"796","volume-title":"Biofilms","author":"Characklis WG","year":"1990"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260411107"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"4634","DOI":"10.1128\/aem.60.12.4634-4637.1994","article-title":"Trichloroethylene removal and oxidation toxicity mediated by toluene dioxygenase of Pseudomonas putida","volume":"60","author":"Heald S","year":"1994","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(98)00043-8"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.58.6.1839-1846.1992"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02919003"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0290(20000805)69:3<256::AID-BIT3>3.0.CO;2-2"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)90827-3"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(05)81005-8"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"3479","DOI":"10.1128\/aem.61.9.3479-3481.1995","article-title":"Induction of toluene oxidation activity in Pseudomonas mendocina KR1 and Pseudomonas sp. strain ENVPC5 by chlorinated solvents and alkanes","volume":"61","author":"McClay K","year":"1995","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02920477"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1128\/aem.37.5.878-885.1979","article-title":"Comparative effects of AroClor 1254 (polychlorinated biphenyls) and phenanthrene on glucose uptake by freshwater microbial populations","volume":"37","author":"Sayler GS","year":"1979","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_1_19_1","unstructured":"ShingletonJT.1998.Degradation of toluene and TCE in a radial flow reactor. PhD thesis Department of Chemical Engineering University of Tennessee."},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"5049","DOI":"10.1128\/AEM.64.12.5049-5052.1998","article-title":"Induction of the tod operon by trichloroethylene in Pseudomona putida TVA8","volume":"64","author":"Shingleton JT","year":"1998","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_1_21_1","first-page":"479","volume-title":"Bioprocess engineering","author":"Shuler ML","year":"1992"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-43-2-159"},{"key":"e_1_2_1_23_1","first-page":"784","volume-title":"Manual of environmental microbiology","author":"Wackett LP","year":"1997"},{"key":"e_1_2_1_24_1","unstructured":"YuF.1998.Experimental design and mathematical modeling of trichloroethylene cometabolism in a packed bed biofilm reactor byPseudomonas putidaF1. 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