{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:48:47Z","timestamp":1760060927762,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,11,12]],"date-time":"2001-11-12T00:00:00Z","timestamp":1005523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp;amp; Bioengineering"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A membrane enzyme reactor consisting of variable pieces of replaceable cell\u2010immobilized membranes was proposed for the continuous production of bioproducts. To demonstrate the characteristics of the reactor, cell\u2010immobilized membranes were prepared by the entrapment of permeabilized recombinant <jats:italic>Escherichia coli<\/jats:italic> cells containing penicillin G acylase within the gluten matrices. A stainless\u2010steel net that was created with a mesh frame was used to support each gluten membrane so that the membranes could be filled into the rectangular\u2010shaped reactor. The reactor equipped with either six or 12 pieces of cell\u2010immobilized gluten membranes containing a biomass concentration of 5%, w\/w was effective in catalyzing the production of 6\u2010aminopenicillanic acid from penicillin G. In comparison with intact cells, the cell\u2010immobilized preparation was more stable and the half\u2010life time of the immobilized cell\u2010associate enzyme in gluten membrane was estimated to be 36 days by a long\u2010term operation. As the substrate solution was forced to flow through the reactor equipped with six membranes and in the direction perpendicular to the membranes, the pressure drop was determined to be less than 50 cm H<jats:sub>2<\/jats:sub>O with a flow\u2010rate up to 50 mL\/min. This low pressure due to the porous structure of gluten membrane would lead to a lower operational cost. Increasing either the number of membranes or the area of each cell\u2010immobilized membrane can easily do scaling\u2010up of this membrane reactor. \u00a9 2001 John Wiley &amp; Sons, Inc. Biotechnol Bioeng 76: 311\u2013317, 2001.<\/jats:p>","DOI":"10.1002\/bit.10100","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T17:17:12Z","timestamp":1030295832000},"page":"311-317","source":"Crossref","is-referenced-by-count":1,"title":["A novel enzyme reactor using gluten membrane entrapping cell\u2010associated enzyme"],"prefix":"10.1002","volume":"76","author":[{"given":"Wen\u2010Chien","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shang\u2010Hsin","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,11,12]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260360312"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(72)90027-7"},{"key":"e_1_2_1_4_1","first-page":"457","article-title":"Formulation of a pencillin electrode by immobilizing the penicillinase in gluten membrane","volume":"30","author":"Chao H\u2010P","year":"1999","journal-title":"J Chin Inst Chem Eng"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/BA20000003"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1389-1723(99)80161-3"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19990505)63:3<263::AID-BIT2>3.0.CO;2-T"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(00)00298-2"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-2509(53)80001-1"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.280510103"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.280580105"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(92)90124-7"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02788005"},{"key":"e_1_2_1_14_1","first-page":"95","volume-title":"Advances in chemical engineering, Vol. 4","author":"Levenspiel O","year":"1963"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/10242420009003634"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00154316"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00129388"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0734-9750(00)00039-2"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(94)90030-2"},{"key":"e_1_2_1_20_1","first-page":"236","volume-title":"Principles of adsorption and adsorption processes","author":"Ruthvan DM","year":"1984"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.10100","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.10100","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:22:20Z","timestamp":1760037740000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.10100"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,11,12]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,12]]}},"alternative-id":["10.1002\/bit.10100"],"URL":"https:\/\/doi.org\/10.1002\/bit.10100","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"type":"print","value":"0006-3592"},{"type":"electronic","value":"1097-0290"}],"subject":[],"published":{"date-parts":[[2001,11,12]]}}}