{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T12:44:57Z","timestamp":1761309897522,"version":"build-2065373602"},"reference-count":21,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,5,21]],"date-time":"2002-05-21T00:00:00Z","timestamp":1021939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Economic Affairs from The Netherlands","award":["IOP Katalyse","IKA94026B (Innovation Oriented Research Program on Catalysis)"],"award-info":[{"award-number":["IOP Katalyse","IKA94026B (Innovation Oriented Research Program on Catalysis)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2002,7,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A relatively new hydroxynitrile lyase\u2010catalyzed reaction was optimized to be suitable for rapid and efficient development of a full\u2010scale production process. The conversion of 4\u2010hydroxybenzaldehyde into (<jats:italic>R<\/jats:italic>)\u20104\u2010hydroxymandelonitrile, catalyzed by <jats:italic>Prunus amygdalus<\/jats:italic> hydroxynitrile lyase, was carried out in a biphasic system of aqueous buffer (pH 5.5) and methyl <jats:italic>tert<\/jats:italic>\u2010butyl ether and is described with a process model. The process model consists of a description of the reaction kinetics, mass transfer kinetics, and mass balances for both the aqueous and the organic phase. Values are determined for the equilibrium constant, the enzyme kinetic parameters, the lumped mass transfer coefficient for benzaldehyde, and the partition coefficients. By using estimated prices of enzyme and reactor use, the optimum aqueous phase volume fraction and required enzyme concentration were calculated at a temperature of 20\u00b0C for a batch\u2010operated stirred tank reactor. According to the process model it was possible to convert 90% of the 4\u2010hydroxybenzaldehyde into (<jats:italic>R<\/jats:italic>)\u20104\u2010hydroxymandelonitrile with 95% enantiomeric excess. The price optimum for this reaction was found at an aqueous phase volume of 17% of the total volume. The required enzyme concentration to meet the targets was 28.6 g\/L aqueous phase. At the predicted optimum, the synthesis was performed experimentally and the results were in accordance with the simulation regarding the extent of conversion and the enantiomeric excess. \u00a9 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 79: 154\u2013164, 2002.<\/jats:p>","DOI":"10.1002\/bit.10317","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T08:21:52Z","timestamp":1033892512000},"page":"154-164","source":"Crossref","is-referenced-by-count":9,"title":["Development of (<i>R<\/i>)\u20104\u2010hydroxymandelonitrile synthesis in an aqueous\u2013organic biphasic stirred tank batch reactor"],"prefix":"10.1002","volume":"79","author":[{"given":"W. F.","family":"Willeman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Neuhofer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Wirth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"P\u00f6chlauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J. J.","family":"Straathof","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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