{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:44:10Z","timestamp":1791254650864,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,8,9]],"date-time":"2002-08-09T00:00:00Z","timestamp":1028851200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp;amp; Bioengineering"],"published-print":{"date-parts":[[2002,10,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nutrient and oxygen supply of cells are crucial to tissue engineering in general. If a sufficient supply cannot be maintained, the development of the tissue will slow down or even fail completely. Previous studies on oxygen supply have focused on measurement of oxygen partial pressures (pO<jats:sub>2<\/jats:sub>) in culture media or described the use of invasive techniques with spatially limited resolution. The experimental setup described here allows for continuous, noninvasive, high\u2010resolution pO<jats:sub>2<\/jats:sub> measurements over the cross\u2010section of cultivated tissues. Applying a recently developed technique for time\u2010resolved pO<jats:sub>2<\/jats:sub> sensing using optical sensor foils, containing luminescent O<jats:sub>2<\/jats:sub>\u2010sensitive indicator dyes, we were able to monitor and analyze gradients in the oxygen supply in a tissue over a 3\u2010week culture period. Cylindrical tissue samples were immobilized on top of the sensors. By measuring the luminescence decay time, two\u2010dimensional pO<jats:sub>2<\/jats:sub> distributions across the tissue section in contact with the foil surface were determined. We applied this technique to cartilage explants and to tissue\u2010engineered cartilage. For both tissue types, changes were detected in monotonously decreasing gradients of pO<jats:sub>2<\/jats:sub> from the surface with high pO<jats:sub>2<\/jats:sub> to minimum pO<jats:sub>2<\/jats:sub> values in the center of the samples. Nearly anoxic conditions were observed in tissue constructs (\u223c0 Torr) but not in excised cartilage discs (\u223c20 Torr) after 1 day. Furthermore, the oxygen supply seemed to strongly depend on cell density and cell function. Additionally, histological analysis revealed a maximum depth of \u223c1.3 mm of regular cartilage development in constructs grown under the applied culture conditions. Correlating analytical and histological analysis with the oxygen distributions, we found that pO<jats:sub>2<\/jats:sub> values below 11 Torr might impair proper tissue development in the center. The results illustrate that the method developed is an ideal one to precisely assess the oxygen demand of cartilage cultures. \u00a9 2002 Wiley Periodicals, Inc. 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