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Reduction rates were metal specific with the following rate trend: Fe(III)citrate \u2265 Fe(III)NTA &gt; Co(III)EDTA<jats:sup>\u2212<\/jats:sup> &gt; UO<jats:sub>2<\/jats:sub><jats:sup>2+<\/jats:sup> &gt; CrO<jats:sub>4<\/jats:sub><jats:sup>2\u2212<\/jats:sup> &gt; TcO<jats:sub>4<\/jats:sub><jats:sup>\u2212<\/jats:sup>, except for CrO<jats:sub>4<\/jats:sub><jats:sup>2\u2212<\/jats:sup> when H<jats:sub>2<\/jats:sub> was used as electron donor. The metal reduction rates were also electron donor dependent with faster rates observed for H<jats:sub>2<\/jats:sub> than lactate<jats:sup>\u2212<\/jats:sup> for all <jats:italic>Shewanella<\/jats:italic> species despite higher initial lactate (10 m<jats:italic>M<\/jats:italic>) than H<jats:sub>2<\/jats:sub> (0.48 m<jats:italic>M<\/jats:italic>). The bioreduction of CrO<jats:sub>4<\/jats:sub><jats:sup>2\u2212<\/jats:sup> was anomalously slower compared to the other metals with H<jats:sub>2<\/jats:sub> as an electron donor relative to lactate and reduction ceased before all the CrO<jats:sub>4<\/jats:sub><jats:sup>2\u2212<\/jats:sup> had been reduced. Transmission electron microscopic (TEM) and energy\u2010dispersive spectroscopic (EDS) analyses performed on selected solids at experiment termination found precipitates of reduced U and Tc in association with the outer cell membrane and in the periplasm of the bacteria. The kinetic rates of metal reduction were correlated with the precipitation of reduced metal phases and their causal relationship discussed. The experimental rate data were well described by a Monod kinetic expression with respect to the electron acceptor for all metals except CrO<jats:sub>4<\/jats:sub><jats:sup>2\u2212<\/jats:sup>, for which the Monod model had to be modified to account for incomplete reduction. However, the Monod models became statistically over\u2010parametrized, resulting in large uncertainties of their parameters. A first\u2010order approximation to the Monod model also effectively described the experimental results, but the rate coefficients exhibited far less uncertainty. The more precise rate coefficients of the first\u2010order model provided a better means than the Monod parameters, to quantitatively compare the reduction rates between metals, electron donors, and DMRB species.  \u00a9 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 80: 637\u2013649, 2002.<\/jats:p>","DOI":"10.1002\/bit.10430","type":"journal-article","created":{"date-parts":[[2002,10,14]],"date-time":"2002-10-14T12:41:24Z","timestamp":1034599284000},"page":"637-649","source":"Crossref","is-referenced-by-count":281,"title":["Reduction kinetics of Fe(III), Co(III), U(VI), Cr(VI), and Tc(VII) in cultures of dissimilatory metal\u2010reducing bacteria"],"prefix":"10.1002","volume":"80","author":[{"given":"Chongxuan","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri A.","family":"Gorby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Zachara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jim K.","family":"Fredrickson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher F.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,10,11]]},"reference":[{"key":"e_1_2_1_2_1","unstructured":"AllisonJD BrownDS Novo\u2010GradacKJ.1991.MINITEQA2\/\/PRODEFA2 A geochemical assessment model for environmental systems (version 3). 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