{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T03:10:44Z","timestamp":1773630644191,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2003,1,16]],"date-time":"2003-01-16T00:00:00Z","timestamp":1042675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2003,3,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The <jats:italic>sec<\/jats:italic>\u2010alcohol dehydrogenase activity of whole cells of <jats:italic>Rhodococcus ruber<\/jats:italic> DSM 44541 has been employed as an efficient biocatalytic redox system due to the use of acetone and 2\u2010propanol at elevated concentrations for cofactor regeneration in the oxidation and reduction mode, respectively, and external addition of NADH\/NAD<jats:sup>+<\/jats:sup> can be omitted. The operational half\u2010life time of the redox system is 29 hours in 20% v\/v acetone and 37 hours in 30% v\/v 2\u2010propanol. The Redox system allows the enantioselective oxidation of <jats:italic>sec<\/jats:italic>\u2010alcohols and the asymmetric reduction of ketones to furnish (<jats:italic>S<\/jats:italic>)\u2010configurated alcohols in high optical purity. The stability of the cells towards further organic solvents was investigated. In addition, the system displays thermostability of up to 60\u00b0C and pH stability of up to pH 11. The system represents a simple to handle tool for environmentally benign redox reactions.  \u00a9 2003 Wiley Periodicals, Inc. 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