{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T10:30:36Z","timestamp":1778668236420,"version":"3.51.4"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T00:00:00Z","timestamp":1047340800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp;amp; Bioengineering"],"published-print":{"date-parts":[[2003,6,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The use of fluidizable affinity adsorbents for the adsorption of cells in expanded mode is investigated. Affinity adsorbents have been synthesized by immobilizing the lectin Concanavalin A onto the surface of triazine\u2010activated perfluorocarbon\u2010solids. The adsorbents were found to adsorb <jats:italic>Saccharomyces cerevisiae<\/jats:italic> cells from solution with adsorption capacities of up to 6.8 \u00d7 10<jats:sup>9<\/jats:sup> cells mL<jats:sup>\u22121<\/jats:sup>. Adsorption kinetics were rapid with a time constant of \u22648 min. The adsorbed cells could be eluted using 500 m<jats:italic>M<\/jats:italic> methyl \u03b1,<jats:sc>D<\/jats:sc>\u2010mannopyranoside, although the kinetics of release were slowed by the multipoint nature of the interaction. The dynamic capacity of the Con A PVA FEP in expanded mode was up to 4.5 \u00d7 10<jats:sup>9<\/jats:sup> cells mL<jats:sup>\u22121<\/jats:sup>. The operating parameters of bed height, application flow rate, and adsorbent size distribution were investigated for any potential improvements in throughput, which may improve utility for more fragile cells. A decrease in settled\u2010bed height from 20 to 5 cm resulted in a decrease in dynamic capacity of 27% from 4.5 to 3.3 \u00d7 10<jats:sup>9<\/jats:sup> cells mL<jats:sup>\u22121<\/jats:sup>. An increase in application flow rate from 0.7 to 2.0 mL\/min<jats:sup>\u22121<\/jats:sup> (resulting in an expansion increase from two\u2010 to fourfold) resulted in a 40% decrease in dynamic capacity from 4.0 to 2.4 \u00d7 10<jats:sup>9<\/jats:sup> cells mL<jats:sup>\u22121<\/jats:sup>. An increase in the mean size distribution of the perfluorocarbon from 42 to 69 \u03bcm and therefore the flow rate needed for twofold expansion of 0.7 to 1.5 mL\/min<jats:sup>\u22121<\/jats:sup> resulted in a 56% decrease in dynamic capacity from 4.0 to 1.8 \u00d7 10<jats:sup>9<\/jats:sup> cells mL<jats:sup>\u22121<\/jats:sup>. The expanded bed, using certain combinations of the operating parameters, therefore shows significant potential for the robust, high efficiency and high capacity capture and separation of cells. \u00a9 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 82: 506\u2013516, 2003.<\/jats:p>","DOI":"10.1002\/bit.10596","type":"journal-article","created":{"date-parts":[[2003,3,18]],"date-time":"2003-03-18T11:38:13Z","timestamp":1047987493000},"page":"506-516","source":"Crossref","is-referenced-by-count":13,"title":["Impact of operating variables on the expanded bed adsorption of <i>Saccharomyces cerevisiae<\/i> cells using a concanavalin A derivatized perfluorocarbon"],"prefix":"10.1002","volume":"82","author":[{"given":"R. H.","family":"Clemmitt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. A.","family":"Chase","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,3,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-198408000-00009"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1182\/blood.V67.2.509.509","article-title":"Elimination of Daudi lymphoblasts from human bone marrow using avidin\u2010biotin immunoadsorption","volume":"67","author":"Berenson RJ","year":"1986","journal-title":"Blood"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008039530302"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-2509(00)00549-2"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1352(199812)11:1\/6<217::AID-JMR426>3.0.CO;2-D"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(20000120)67:2<206::AID-BIT10>3.0.CO;2-X"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(90)82357-2"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(90)82430-9"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(90)82431-0"},{"key":"e_1_2_1_11_1","first-page":"213","volume-title":"Cell separation: A practical approach","author":"Eggleton P","year":"1989"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/masy.19930700133"},{"key":"e_1_2_1_13_1","first-page":"222","volume-title":"Practical handbook of biochemistry and molecular biology","author":"Fasman GD","year":"1989"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19990820)64:4<484::AID-BIT11>3.0.CO;2-Z"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)01308-4"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0032-9592(98)00083-1"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008193312969"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008135528899"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(93)90275-C"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260270509"},{"key":"e_1_2_1_21_1","first-page":"217","article-title":"Analysis of some operating parameters of novel adsorbents for recovery of proteins in expanded beds","volume":"5","author":"Hjorth P","year":"1995","journal-title":"Bioseparation"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(97)01057-3"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19970705)55:1<54::AID-BIT7>3.0.CO;2-W"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)96268-X"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp950055i"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1352(199634\/12)9:5\/6<607::AID-JMR308>3.0.CO;2-S"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260480407"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)00191-X"},{"key":"e_1_2_1_29_1","unstructured":"NashDC.1997.Modified polystyrenic supports for protein separations. PhD Thesis University of Cambridge UK."},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990160105"},{"key":"e_1_2_1_31_1","first-page":"169","volume-title":"Cell separation: A practical approach","author":"Ormerod MG","year":"1999"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19970220)53:4<427::AID-BIT11>3.0.CO;2-D"},{"key":"e_1_2_1_33_1","first-page":"46","volume-title":"Cell separation: A practical approach","author":"Patel D","year":"1998"},{"key":"e_1_2_1_34_1","unstructured":"PitfieldID.1992.Perfluorocarbon chromatographic supports. PhD Thesis University of Cambridge UK."},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(93)90364-D"},{"key":"e_1_2_1_36_1","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.4049\/jimmunol.118.4.1409","article-title":"Isolation and enumeration of peripheral blood monocytes","volume":"118","author":"Sanderson RJ","year":"1977","journal-title":"J Immunol"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(93)90412-Z"},{"key":"e_1_2_1_38_1","series-title":"Laboratory techniques in biochemistry and molecular biology","author":"Sharpe PT","year":"1988"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260400711"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/BFb0103305"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/polc.5070720125"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/masy.19880170113"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-3886(96)90098-3"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.10596","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.10596","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:47:00Z","timestamp":1760035620000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.10596"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,3,11]]},"references-count":42,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2003,6,5]]}},"alternative-id":["10.1002\/bit.10596"],"URL":"https:\/\/doi.org\/10.1002\/bit.10596","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,3,11]]}}}