{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:27:04Z","timestamp":1791160024041,"version":"4.1.0"},"reference-count":8,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2003,3,28]],"date-time":"2003-03-28T00:00:00Z","timestamp":1048809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2003,6,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two suggestions can be found in the literature to improve the reproducibility of the Mandels' filter paper assay: add supplemental cellobiase and increase the boiling time for color development. Here we provide data that strongly supports adding supplemental cellobiase. Adding supplemental cellobiase increased assay response by 56%. Cellulases from different sources have different cellobiase activities, which would cause significant variation in the assay response. There is no need for additional boiling time\u20145 minutes is sufficient. For maximum reproducibility, it is essential that the water bath vigorously boil so that temperature excursions are minimized.  \u00a9 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 82: 745\u2013749, 2003.<\/jats:p>","DOI":"10.1002\/bit.10620","type":"journal-article","created":{"date-parts":[[2003,3,31]],"date-time":"2003-03-31T06:59:06Z","timestamp":1049093946000},"page":"745-749","source":"Crossref","is-referenced-by-count":62,"title":["Suggested improvements to the standard filter paper assay used to measure cellulase activity"],"prefix":"10.1002","volume":"82","author":[{"given":"Guillermo","family":"Coward\u2010Kelly","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cateryna","family":"Aiello\u2010Mazzari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sehoon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cesar","family":"Granda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Holtzapple","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,3,28]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260281109"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.280340305"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1351\/pac198759020257"},{"key":"e_1_2_1_5_1","unstructured":"MandelsM AndreottiR RocheC.1976.Measurement of saccharifying cellulase. In: Enzymatic conversion of cellulosic materials: technology and applications symposium proceedings September 8\u201310 1975 Natick Massachusetts (Editors: Gaden L Mandels M Reese E Spano L) John Wiley & Sons. Biotechnol Bioeng Symp 6:21\u201333."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac60147a030"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260260727"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002530000327"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00268203"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.10620","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.10620","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:39:35Z","timestamp":1760035175000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.10620"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,3,28]]},"references-count":8,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2003,6,20]]}},"alternative-id":["10.1002\/bit.10620"],"URL":"https:\/\/doi.org\/10.1002\/bit.10620","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,3,28]]}}}