{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:36:48Z","timestamp":1773657408028,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,12,31]],"date-time":"2003-12-31T00:00:00Z","timestamp":1072828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2004,2,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Advantages of performing penicillin acylase\u2010catalyzed synthesis of new penicillins and cephalosporins by enzymatic acyl transfer to the \u03b2\u2010lactam antibiotic nuclei in the supersaturated solutions of substrates have been demonstrated. It has been shown that the effective nucleophile reactivity of 6\u2010aminopenicillanic (6\u2010APA) and 7\u2010aminodesacetoxycephalosporanic (7\u2010ADCA) acids in their supersaturated solutions continue to grow proportionally to the nucleophile concentration. As a result, synthesis\/hydrolysis ratio in the enzymatic synthesis can be significantly (up to three times) increased due to the nucleophile supersaturation. In the antibiotic nuclei conversion to the target antibiotic the remarkable improvement (up to 14%) has been gained. Methods of obtaining relatively stable supersaturated solutions of 6\u2010APA, 7\u2010ADCA, and D\u2010p\u2010hydroxyphenylglycine amide (D\u2010HPGA) have been developed and syntheses of ampicillin, amoxicillin, and cephalexin starting from the supersaturated homogeneous solutions of substrates were performed. Higher synthetic efficiency and increased productivity of these reactions compared to the heterogeneous \u201caqueous solution\u2010precipitate\u201d systems were observed. The suggested approach seems to be an effective solution for the aqueous synthesis of the most widely requested \u03b2\u2010lactam antibiotics (i.e., amoxicillin, cephalexin, cephadroxil, cephaclor, etc.). \u00a9 2004 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.10863","type":"journal-article","created":{"date-parts":[[2004,1,23]],"date-time":"2004-01-23T07:01:05Z","timestamp":1074841265000},"page":"323-329","source":"Crossref","is-referenced-by-count":52,"title":["Penicillin acylase\u2010catalyzed synthesis of \u03b2\u2010lactam antibiotics in highly condensed aqueous systems: Beneficial impact of kinetic substrate supersaturation"],"prefix":"10.1002","volume":"85","author":[{"given":"Maxim I.","family":"Youshko","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harold M.","family":"Moody","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander L.","family":"Bukhanov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilhelmus H. 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Mathematic modeling of the process and quantitative characterization of the nucleophile reactivity","volume":"65","author":"Youshko MI","year":"2000","journal-title":"Biokhimiya"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1381-1177(00)00091-6"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.1076"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.10234"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-9639(02)00413-2"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.10863","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.10863","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:52:03Z","timestamp":1760057523000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.10863"}},"subtitle":["Beneficial impact of kinetic substrate supersaturation"],"short-title":[],"issued":{"date-parts":[[2003,12,31]]},"references-count":16,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,2,5]]}},"alternative-id":["10.1002\/bit.10863"],"URL":"https:\/\/doi.org\/10.1002\/bit.10863","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,12,31]]}}}