{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T11:19:36Z","timestamp":1780571976678,"version":"3.54.1"},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2004,4,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rifamycin B is an important polyketide antibiotic used in the treatment of tuberculosis and leprosy. We present results on medium optimization for Rifamycin B production via a barbital insensitive mutant strain of <jats:italic>Amycolatopsis mediterranei<\/jats:italic> S699. Machine\u2010learning approaches such as Genetic algorithm (GA), Neighborhood analysis (NA) and Decision Tree technique (DT) were explored for optimizing the medium composition. Genetic algorithm was applied as a global search algorithm while NA was used for a guided local search and to develop medium predictors. The fermentation medium for Rifamycin B consisted of nine components. A large number of distinct medium compositions are possible by variation of concentration of each component. This presents a large combinatorial search space. Optimization was achieved within five generations via GA as well as NA. These five generations consisted of 178 shake\u2010flask experiments, which is a small fraction of the search space. We detected multiple optima in the form of 11 distinct medium combinations. These medium combinations provided over 600% improvement in Rifamycin B productivity. Genetic algorithm performed better in optimizing fermentation medium as compared to NA. The Decision Tree technique revealed the media\u2013media interactions qualitatively in the form of sets of rules for medium composition that give high as well as low productivity. \u00a9 2004 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.20056","type":"journal-article","created":{"date-parts":[[2004,3,25]],"date-time":"2004-03-25T12:18:32Z","timestamp":1080217112000},"page":"201-208","source":"Crossref","is-referenced-by-count":42,"title":["Optimization of rifamycin B fermentation in shake flasks via a machine\u2010learning\u2010based approach"],"prefix":"10.1002","volume":"86","author":[{"given":"Prashant M.","family":"Bapat","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pramod P.","family":"Wangikar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.10418"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(02)00037-6"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0032-9592(94)87010-1"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8346439"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5439.531"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(67)90147-5"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/scientificamerican0792-66"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jim.2900755"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja9601292"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.10465"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022643204877"},{"key":"e_1_2_1_13_1","unstructured":"RichardWJ LanciniG.1975. Production of rifamycin B. US patent no. 3871965."},{"key":"e_1_2_1_14_1","first-page":"21","article-title":"Production of rifamycins","volume":"6","author":"Sensi P","year":"1967","journal-title":"Prog Ind Microbiol"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.8.2.180"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.55.396"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/PL00009033","article-title":"Production of rifamycin using Amycolatopsis mediterranei (MTCC14)","volume":"20","author":"Venkateswarlu G","year":"1999","journal-title":"Bioproc Eng"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004499900068"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0032-9592(94)00036-0"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M009667200"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.20056","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.20056","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:08:47Z","timestamp":1760036927000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.20056"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,2,19]]},"references-count":19,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,4,20]]}},"alternative-id":["10.1002\/bit.20056"],"URL":"https:\/\/doi.org\/10.1002\/bit.20056","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,2,19]]}}}