{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T13:26:43Z","timestamp":1791034003936,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2004,8,24]],"date-time":"2004-08-24T00:00:00Z","timestamp":1093305600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2004,9,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An on\u2010line biosensor consisting of immobilized <jats:italic>Thiobacillus ferrooxidans<\/jats:italic> and an oxygen electrode was developed for automated monitoring of acute toxicity in water samples. <jats:italic>T. ferrooxidans<\/jats:italic> is an obligatory acidophilic, autotrophic bacterium and derives its energy by the oxidation of ferrous ion, elemental sulfur, and reduced sulfur compounds including metal sulfides. The assay is based on the monitoring of a current increase by addition of toxicoids, which is caused by the inhibition of bacterial respiration and decrease in oxygen consumption. Optimum cell number on the membrane was 5.0 \u00d7 10<jats:sup>8<\/jats:sup> cells. The steady\u2010state current was obtained when concentration of FeSO<jats:sub>4<\/jats:sub> was above 3.6 m<jats:italic>M<\/jats:italic> at pH 3. The sensor response of <jats:italic>T. ferrooxidans<\/jats:italic> immobilized membrane for 5.0 \u03bc<jats:italic>M<\/jats:italic> KCN was within an error of 10% for 30 membranes. A linear relationship was obtained at KCN concentration in the range of 0.5\u20133.0 \u03bc<jats:italic>M<\/jats:italic> in a flow\u2010type monitoring system. Minimum detectable concentrations of KCN, Na<jats:sub>2<\/jats:sub>S, and NaN<jats:sub>3<\/jats:sub> were 0.5, 1.2, and 0.07 \u03bc<jats:italic>M<\/jats:italic>, respectively. The monitoring system contained two biosensors and these sensors were cleaned with sulfuric acid (pH 1.5) twice a day. This treatment could remove fouling on microbial immobilized membrane by natural water and ferrous precipitation in the flow cell. This flow\u2010type monitoring sensor was operated continuously for 5 months. Also, <jats:italic>T. ferrooxidans<\/jats:italic> immobilized membrane can be stored for one month at 4\u00b0C when preserved with wet absorbent cotton under argon gas. \u00a9 2004 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.20193","type":"journal-article","created":{"date-parts":[[2004,8,25]],"date-time":"2004-08-25T03:03:41Z","timestamp":1093403021000},"page":"905-911","source":"Crossref","is-referenced-by-count":22,"title":["Development of an automated water toxicity biosensor using <i>Thiobacillus ferrooxidans<\/i> for monitoring cyanides in natural water for a water filtering plant"],"prefix":"10.1002","volume":"87","author":[{"given":"Mina","family":"Okochi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Mima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maki","family":"Miyata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youhei","family":"Shinozaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Haraguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minoru","family":"Fujisawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masao","family":"Kaneko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadashi","family":"Masukata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadashi","family":"Matsunaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,8,24]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0956-5663(01)00303-7"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"2922","DOI":"10.1128\/aem.56.9.2922-2923.1990","article-title":"Thiobacillus ferrooxidans, a facultative hydrogen oxidizer","volume":"56","author":"Drobner E","year":"1990","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002160100925"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0956-5663(01)00321-9"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/elan.1140081005"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1656(96)01399-5"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4173(82)90007-6"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"271","DOI":"10.2166\/wst.2002.0603","article-title":"Application of toxicity monitor using nitrifying bacteria biosensor to sewerage systems","volume":"45","author":"Inui T","year":"2002","journal-title":"Water Sci Technol"},{"key":"e_1_2_1_10_1","first-page":"95","volume-title":"Bioindicators and environmental management","author":"Karmer KJM","year":"1991"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260191010"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19990405)63:1<79::AID-BIT8>3.0.CO;2-Z"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.34.100180.001403"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/es00027a010"},{"key":"e_1_2_1_15_1","unstructured":"NakamuraE TanakaH TanakaY HoshikawaH KatoT.1990. 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