{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:40:12Z","timestamp":1789180812481,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,9,21]],"date-time":"2004-09-21T00:00:00Z","timestamp":1095724800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2004,11,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two biokinetic models employing the Michaelis\u2010Menten equation for anaerobic reductive dechlorination of tetrachloroethylene (PCE) and trichloroethylene (TCE) were developed. The models were compared with results from batch kinetic tests conducted over a wide range of PCE and TCE concentrations with two different dechlorinating cultures. One model applies Michaelis\u2010Menten kinetics with competitive inhibition among chlorinated aliphatic hydrocarbons (CAHs), while the other model includes both competitive inhibition and Haldane inhibition at high CAH concentrations. Model simulations with competitive inhibition simulated the experimental results well for PCE concentrations lower than 300 \u03bc<jats:italic>M<\/jats:italic>. However, simulations deviated from the experimental observations for PCE or TCE concentrations greater than 300\u2013400 \u03bc<jats:italic>M<\/jats:italic>. The kinetic model that incorporated both competitive and Haldane inhibitions better simulated experimental data for PCE concentrations near the solubility limit (1000 \u03bc<jats:italic>M<\/jats:italic>), and TCE concentrations at half its solubility limit (4000 \u03bc<jats:italic>M<\/jats:italic>). Based on the modeling analysis of the experimental results, the PM culture (Point Mugu, CA) had very high Haldane inhibition constants for <jats:italic>cis<\/jats:italic>\u20101,2\u2010dichlororethylene (<jats:italic>c<\/jats:italic>\u2010DCE) and vinyl chloride (VC) (6000 and 7000 \u03bc<jats:italic>M<\/jats:italic>, respectively), indicating very weak Haldane inhibition, while the EV culture (the Evanite site in Corvallis, OR) had lower Haldane inhibition constants for TCE, <jats:italic>c<\/jats:italic>\u2010DCE, and VC of 900, 750, and 750 \u03bc<jats:italic>M<\/jats:italic>, respectively. The BM culture (a binary mixed culture of the PM and EV cultures) had transformation abilities that represented the mixture of the EV and PM cultures. Model simulations of the BM culture transformation abilities were well represented by separate rate equations and model parameters for the two independent cultures that were simultaneously solved. Modeling results indicated that a combination of competitive and Haldane inhibition kinetics is required to simulate dechlorination over a broad range of concentrations up to the solubility limit of PCE and half the solubility limit of TCE. \u00a9 2004 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.20260","type":"journal-article","created":{"date-parts":[[2004,9,22]],"date-time":"2004-09-22T03:20:07Z","timestamp":1095823207000},"page":"451-464","source":"Crossref","is-referenced-by-count":93,"title":["Kinetics and modeling of reductive dechlorination at high PCE and TCE concentrations"],"prefix":"10.1002","volume":"88","author":[{"given":"Seungho","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lewis","family":"Semprini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,9,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1061\/(ASCE)0733-9372(1998)124:11(1076)"},{"key":"e_1_2_1_3_1","volume-title":"Biochemical engineering fundamentals","author":"Bailey J","year":"1986"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.2.953-959.2003"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/es0348647"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/es990638e"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1139\/m97-122"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/es980136l"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19980320)57:6<751::AID-BIT13>3.0.CO;2-A"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/es9807690"},{"key":"e_1_2_1_11_1","unstructured":"HastonZC.1999.Factors affecting growth and utilization in the anaerobic dehalogenation of chlorinated ethenes. Ph.D. thesis Stanford University."},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1128\/AEM.68.2.485-495.2002","article-title":"Molecular analysis of Dehalococcoides 16S ribosomal DNA from chloroethene\u2010contaminated sites through North America and Europe","volume":"68","author":"Hendrickson ER","year":"2002","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.4.1369-1374.2000"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.8.4628-4638.2003"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/es0255711"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.1126\/science.276.5318.1568","article-title":"Isolation of bacterium that reductively dechlorinates tetrachloroethene to ethene","volume":"276","author":"Maymo\u2010Gatell X","year":"1997","journal-title":"Science"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/es026352i"},{"key":"e_1_2_1_18_1","first-page":"37","volume-title":"In\u2010situ bioremediation","author":"Rittmann BE","year":"1994"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0290(20001120)70:4<436::AID-BIT9>3.0.CO;2-6"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/es00054a033"},{"key":"e_1_2_1_21_1","first-page":"591","volume-title":"Proceedings of the Fourth International Conference on In Situ and On\u2010Site Bioremediation","author":"Tonnaer H","year":"1997"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1551-8833.1984.tb05334.x"},{"key":"e_1_2_1_23_1","volume-title":"Chemical properties handbook: Physical, thermodynamics, environmental, transport, safety, and health related properties for organic and inorganic chemicals","author":"Yaws CL","year":"1999"},{"key":"e_1_2_1_24_1","unstructured":"YuS.2003.Kinetics and modeling investigations of the anaerobic reductive dechlorination of chlorinated ethylenes using single and binary mixed cultures and silicon\u2010based organic compounds as slow\u2010release substrates. Ph.D. thesis Oregon State University Corvallis."},{"key":"e_1_2_1_25_1","first-page":"2B","volume-title":"Proceedings of the Third International Conference on Remediation of Chlorinated and Recalcitrant Compounds","author":"Yu S","year":"2002"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1354(02)00222-1"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.20260","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.20260","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:07:50Z","timestamp":1760058470000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.20260"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,9,21]]},"references-count":25,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,11,20]]}},"alternative-id":["10.1002\/bit.20260"],"URL":"https:\/\/doi.org\/10.1002\/bit.20260","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,9,21]]}}}