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Initially (i.e., until approximately day 80), the H<jats:sub>2<\/jats:sub>\u2010fed bioreactor showed the best ability to completely dechlorinate the dosed PCE (0.5 mmol\/L) to ethene whereas, in the presence of methanol, butyric acid or no electron donor added (but low\u2010level yeast extract), dechlorination was limited by the fermentation of the organic substrates and in turn by H<jats:sub>2<\/jats:sub> availability. As the study progressed, the H<jats:sub>2<\/jats:sub>\u2010fed reactor experienced a diminishing ability to dechlorinate, while more stable dechlorinating activity was maintained in the reactors that were fed organic donors. The initial diminished ability of the H<jats:sub>2<\/jats:sub>\u2010fed reactor to dechlorinate (after about 100 days), could be partially explained in terms of increased competition for H<jats:sub>2<\/jats:sub> between dechlorinators and methanogens, whereas other factors such as growth\u2010factor limitation and\/or accumulation of toxic and\/or inhibitory metabolites were shown to play a role for longer incubation periods (over 500 days). In spite of decreasing activity with time, the H<jats:sub>2<\/jats:sub>\u2010fed reactor proved to be the most effective in PCE dechlorination: after about 500 days, more than 65% of the added PCE was dechlorinated to ethene in the H<jats:sub>2<\/jats:sub>\u2010fed reactor, versus 36%, 22%, and &lt;1% in the methanol\u2010fed, butyrate\u2010fed, and control reactors, respectively. \u00a9 2005 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.20569","type":"journal-article","created":{"date-parts":[[2005,7,8]],"date-time":"2005-07-08T13:21:04Z","timestamp":1120828864000},"page":"743-753","source":"Crossref","is-referenced-by-count":69,"title":["Comparative study of methanol, butyrate, and hydrogen as electron donors for long\u2010term dechlorination of tetrachloroethene in mixed anerobic cultures"],"prefix":"10.1002","volume":"91","author":[{"given":"Federico","family":"Aulenta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James M.","family":"Gossett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Petrangeli","family":"Papini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Rossetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro","family":"Majone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,7,8]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022868712613"},{"key":"e_1_2_1_3_1","unstructured":"AulentaF GiarolliF MajoneM TandoiV Petrangeli PapiniM BeccariM.2003.Reductive dechlorination of tetrachloroethene (PCE) to ethane (ETH) in anaerobic sequencing batch biofilm reactor. 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