{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:54:17Z","timestamp":1760061257124,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,11,28]],"date-time":"2005-11-28T00:00:00Z","timestamp":1133136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp;amp; Bioengineering"],"published-print":{"date-parts":[[2006,2,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The long\u2010term operation of bioremediation technologies relies on the success of the contaminant\u2010degrading microorganism(s) to compete for available resources with microorganisms already present in an aquifer or those that may contaminate a bioreactor. Though research has been performed studying the interaction of multiple species in batch and chemostat reactors, little work has been done looking at multi\u2010species interactions in environments that more closely resemble field\u2010scale applications. The research presented herein examined the interaction of <jats:italic>Burkholderia cepacia<\/jats:italic> PR1\u2010pTOM<jats:sub>31c<\/jats:sub>, an aerobic trichloroethylene (TCE)\u2010degrading bacterium, with <jats:italic>Klebsiella oxytoca<\/jats:italic>, a facultative bacterium, in a flow\u2010through porous media (PM) reactor. Growth characteristics and population distributions in PM were compared to previously reported values from batch and chemostat reactors. The faster growing organism in batch experiments (<jats:italic>K. oxytoca<\/jats:italic>) did not always have the greater population density in dual\u2010species PM experiments. The biofilm population distribution was influenced by substrate concentration, with <jats:italic>B. cepacia<\/jats:italic> having a greater dual\u2010species population density than <jats:italic>K. oxytoca<\/jats:italic> at a low (30 mg\/L dissolved organic carbon [DOC]) substrate concentration and <jats:italic>K. oxytoca<\/jats:italic> having a greater population density at a high (700 mg\/L DOC) substrate concentration. This change in species population distribution with change in substrate concentration, which was not observed in batch reactors, was also observed in chemostat reactors. Therefore, manipulation of substrate concentration enabled the control of species dominance to the advantage of the TCE degrading population in this dual\u2010species PM system and may provide a mechanism to enhance bioremediation scenarios involving TCE or other contaminants of concern. \u00a9 2005 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.20727","type":"journal-article","created":{"date-parts":[[2005,11,28]],"date-time":"2005-11-28T22:16:51Z","timestamp":1133216211000},"page":"434-442","source":"Crossref","is-referenced-by-count":4,"title":["Effect of substrate concentration on dual\u2010species biofilm population densities of <i>Klebsiella oxytoca<\/i> and <i>Burkholderia cepacia<\/i> in porous media"],"prefix":"10.1002","volume":"93","author":[{"given":"John","family":"Komlos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfred B.","family":"Cunningham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne K.","family":"Camper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert R.","family":"Sharp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1,5]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.57.7.1974-1979.1991"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/08927019209386212"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/08927019109378159"},{"key":"e_1_2_1_5_1","first-page":"325","volume-title":"The microbiology of the terrestrial deep subsurface","author":"Cunningham AB","year":"1997"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1354(97)00281-9"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002489901003"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ep.10003"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-003-1066-z"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.54.6.1365-1372.1988"},{"volume-title":"Brock: Biology of microorganisms","year":"1997","author":"Madigan MT","key":"e_1_2_1_11_1"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00429648"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"2432","DOI":"10.1128\/aem.63.6.2432-2438.1997","article-title":"Impact of nutrient composition on a degradative biofilm community","volume":"63","author":"M\u00f8ller S","year":"1997","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-0611-6_7"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.58.12.3977-3983.1992"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260370813"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002489900002"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0043-1354(94)90089-2"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0043-1354(89)90193-0"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp9600243"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.20727","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.20727","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:36:29Z","timestamp":1760045789000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.20727"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,1,5]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,2,20]]}},"alternative-id":["10.1002\/bit.20727"],"URL":"https:\/\/doi.org\/10.1002\/bit.20727","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"type":"print","value":"0006-3592"},{"type":"electronic","value":"1097-0290"}],"subject":[],"published":{"date-parts":[[2006,1,5]]}}}