{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T18:34:20Z","timestamp":1790361260281,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,3,2]],"date-time":"2006-03-02T00:00:00Z","timestamp":1141257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2006,7,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present contribution is dedicated to experimental and theoretical assessment of microbiological process heterogeneities of the high\u2010pressure (HP) inactivation of <jats:italic>Lactococcus lactis<\/jats:italic> ssp. <jats:italic>cremoris<\/jats:italic> MG 1363. The inactivation kinetics are determined in dependence of pressure, process time, temperature and absence or presence of co\u2010solutes in the buffer system namely 4 M sodium chloride and 1.5 M sucrose. The kinetic analysis is carried out in a 0.1\u2010L autoclave in order to minimise thermal and convective effects. Upon these data, a deterministic inactivation model is formulated with the logistic equation. Its independent variables represent the counts of viable cells (viable but injured) and of the stress\u2010resistant cells (viable and not injured). This model is then coupled to a thermo\u2010fluiddynamical simulation method, high\u2010pressure computer fluid dynamics technique (HP\u2010CFD), which yields spatiotemporal temperature and flow fields occurring during the HP application inside any considered autoclave. Besides the thermo\u2010fluiddynamic quantities, the coupled model predicts also the spatiotemporal distribution of both viable (VC) and stress\u2010resistant cell counts (SRC). In order to assess the process non\u2010uniformity of the microbial inactivation in a 3.3\u2010L autoclave experimentally, microbial samples are placed at two distinct locations and are exposed to various process conditions. It can be shown with both, experimental and theoretical models that thermal heterogeneities induce process non\u2010uniformities of more than one decimal power in the counts of the viable cells at the end of the treatment. \u00a9 2006 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.20852","type":"journal-article","created":{"date-parts":[[2006,3,2]],"date-time":"2006-03-02T20:46:36Z","timestamp":1141332396000},"page":"655-666","source":"Crossref","is-referenced-by-count":13,"title":["Experimental and numerical study of heterogeneous pressure\u2010temperature\u2010induced lethal and sublethal injury of <i>Lactococcus Lactis<\/i> in a medium scale high\u2010pressure autoclave"],"prefix":"10.1002","volume":"94","author":[{"given":"K.V.","family":"Kilimann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Kitsubun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Delgado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.G.","family":"G\u00e4nzle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Chapleau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Le Bail","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Hartmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,3,2]]},"reference":[{"key":"e_1_2_1_2_1","unstructured":"Anonymous.2003.Scientific criteria to ensure safe food. 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