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This strategy is based on site\u2010specific integration of antibody genes in CHO cells, using the FRT\/Flp\u2010In recombinase system. This allows integration of the expression construct at the same genomic site in the host cells, thereby reducing genomic position effects. Different bioreactor batches of Sym001 displayed highly consistent manufacturing yield, antibody composition, binding potency, and functional activity. The results demonstrate that diverse recombinant human polyclonal antibody compositions can be reproducibly generated under conditions directly applicable to industrial manufacturing settings and present a first recombinant polyclonal antibody which could be used for treatment of hemolytic disease of the newborn and\/or idiopathic thrombocytopenic purpura. \u00a9 2006 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.20865","type":"journal-article","created":{"date-parts":[[2006,4,4]],"date-time":"2006-04-04T21:16:21Z","timestamp":1144185381000},"page":"396-405","source":"Crossref","is-referenced-by-count":77,"title":["Production of target\u2010specific recombinant human polyclonal antibodies in mammalian cells"],"prefix":"10.1002","volume":"94","author":[{"given":"Finn C.","family":"Wiberg","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00f8ren 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