{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:33:00Z","timestamp":1786977180328,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,21]],"date-time":"2006-07-21T00:00:00Z","timestamp":1153440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp;amp; Bioengineering"],"published-print":{"date-parts":[[2006,10,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Many superfund sites are currently co\u2010contaminated with organic pollutants such as trichloroethene (TCE) and heavy metals. A promising strategy to address these mixed\u2010waste situations is the use of TCE\u2010degrading rhizobacteria that will survive and thrive in soil heavily polluted with heavy metals. In this work, a gene coding for the metal\u2010binding peptide, EC20, was introduced into rhizobacteria engineered for TCE degradation, resulting in strains with both metal accumulation and TCE degradation capabilities. EC20 was displayed onto the cell surface of\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    strain Pb2\u20101 and\n                    <jats:italic>Rhizobium<\/jats:italic>\n                    strain 10320D using an ice\u2010nucleation protein (INP) anchor. Expression of EC20 was confirmed by Western blot analysis and cells with EC20 expression showed sixfold higher cadmium accumulation than non\u2010engineered strains in the presence of 16 \u00b5M CdCl\n                    <jats:sub>2<\/jats:sub>\n                    . As expected, the TCE degradation rate was reduced in the presence of cadmium for cells without EC20 expression. However, expression of EC20 (higher cadmium accumulation) completely restored the level of TCE degradation. These results demonstrated that EC20 expression enhanced not only cadmium accumulation but also reduced the toxic effect of cadmium on TCE degradation. We expect that similar improvements will be observed when these engineered rhizobacteria are inoculated onto plant roots. \u00a9 2006 Wiley Periodicals, Inc.\n                  <\/jats:p>","DOI":"10.1002\/bit.20950","type":"journal-article","created":{"date-parts":[[2006,7,21]],"date-time":"2006-07-21T21:16:26Z","timestamp":1153516586000},"page":"399-403","source":"Crossref","is-referenced-by-count":38,"title":["Engineering TCE\u2010degrading rhizobacteria for heavy metal accumulation and enhanced TCE degradation"],"prefix":"10.1002","volume":"95","author":[{"given":"Wonkyu","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas K.","family":"Wood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilfred","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/es990809f"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/es00049a001"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0290(20001205)70:5<518::AID-BIT6>3.0.CO;2-5"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0162-0134(01)00392-0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.2.344-349.2002"},{"key":"e_1_2_1_7_1","unstructured":"Environmental health center.1997.Trichloroehtylene (C2HCl3) chemical backgrounder."},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"2802","DOI":"10.1128\/AEM.65.6.2802-2804.1999","article-title":"High\u2010efficiency transformation of Rhizobium leguminosarum by electroporation","volume":"65","author":"Grag B","year":"1999","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1128\/CDLI.6.4.499-503.1999","article-title":"Cell surface display of human immunodeficiency virus type 1 gp120 on Escherichia coli by using ice nucleation protein","volume":"6","author":"Kwak YD","year":"1999","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90533-9"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00164512"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5318.1521"},{"key":"e_1_2_1_13_1","first-page":"383","article-title":"Aerobic metabolism of trichloroethylene by a bacterial isolate","volume":"52","author":"Nelson MJK","year":"1986","journal-title":"Appl Microbiol Biotechnol"},{"key":"e_1_2_1_14_1","unstructured":"OchsnerJC BlackwoodTR MichelettiWC.1979.Status assessment of toxic chemicals: Trichloroethylene. 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Presented at the batelle 2nd international conference on remediation of chlorinated and recalcitrant compounds Monterey CA."},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.5.2107-2115.2001"},{"key":"e_1_2_1_26_1","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1128\/AEM.64.1.112-118.1998","article-title":"Rhizoremediation of trichloroethylene by a recombinant root\u2010colonizing Pseudomonas fluorescens strain expressing toluene ortho\u2010monooxygenase constitutively","volume":"64","author":"Yee DC","year":"1998","journal-title":"Appl Environ Microbiol"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.20950","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.20950","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:31:14Z","timestamp":1760038274000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.20950"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,7,21]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,10,20]]}},"alternative-id":["10.1002\/bit.20950"],"URL":"https:\/\/doi.org\/10.1002\/bit.20950","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,7,21]]}}}