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The recycling of the required nicotinamide cofactor (NADH) was achieved in a coupled\u2010substrate process. In the course of the reaction the ketone is reduced to the alcohol and the hydrogen donor 2\u2010propanol is oxidized to acetone by one enzyme. This leads to a thermodynamic equilibrium between all four components determining the maximum achievable conversion. To overcome this limitation an in situ product removal technique (ISPR) for the application with whole cells was developed. In this method the most volatile compound is separated from the reaction vessel by an air flow resulting in a shift of the equilibrium towards the desired secondary alcohol. The so\u2010called stripping process represents a simple and efficient method to overcome the thermodynamic limitation in biocatalytic reactions. Employing this method, the conversion of selected biotransformations was increased up to completeness. \u00a9 2006 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.21014","type":"journal-article","created":{"date-parts":[[2006,6,27]],"date-time":"2006-06-27T22:35:28Z","timestamp":1151447728000},"page":"192-198","source":"Crossref","is-referenced-by-count":62,"title":["Overcoming the thermodynamic limitation in asymmetric hydrogen transfer reactions catalyzed by whole cells"],"prefix":"10.1002","volume":"95","author":[{"given":"Katja","family":"Goldberg","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Edegger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Kroutil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Liese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,6,27]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcatb.2004.08.006"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ejic.200300685"},{"key":"e_1_2_1_4_1","volume-title":"Fundamentals of enzyme kinetics","author":"Cornish\u2010Bowden A","year":"1995"},{"key":"e_1_2_1_5_1","first-page":"149","article-title":"Regio\u2010 and stereoselective reduction of diones and oxidation of diols via biocatalytic hydrogen transfer","volume":"6","author":"Edegger K","year":"2006","journal-title":"Eur J Org Chem"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-18537-3"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-002-0936-5"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/op020023t"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/c39720000856"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2003.10.019"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/9783527614165"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/p29860000805"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cite.200500050"},{"key":"e_1_2_1_14_1","volume-title":"Biotechnology","author":"Peters J","year":"1998"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1976.tb10108.x"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.198505391"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3773(20020315)41:6<1014::AID-ANIE1014>3.0.CO;2-6"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo026216w"},{"key":"e_1_2_1_19_1","unstructured":"StampferW KosjekB KroutilW FaberK NiehausF EckJ.2005.Alcohol dehydrogenases with high solvent and temperature stability. 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