{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T03:00:03Z","timestamp":1791169203877,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,8,25]],"date-time":"2006-08-25T00:00:00Z","timestamp":1156464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2006,12,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>As the market for biopharmaceuticals especially monoclonal antibodies (MAbs) rapidly grows, their manufacturing methods are coming under increasing regulatory scrutiny, particularly due to concerns about the potential introduction of adventitious agents from animal\u2010sourced components in the media used for their production in mammalian cell culture. Chinese hamster ovary (CHO) cells are by far the most commonly used production vehicles for these recombinant glycoproteins. In developing animal\u2010component free media for CHO and other mammalian cell lines, the iron\u2010transporter function of serum or human\/bovine transferrin is usually replaced by certain organic or inorganic chelators capable of delivering iron for cell respiration and metabolism, but few of them are sufficiently effective. Selenium is a well\u2010known essential trace element (TE) for cell growth and development, and its positive role in biological system includes detoxification of free radicals by activating glutathione peroxidase. In cell culture, selenium in the form of selenite can help cells to detoxify the medium thus protect them from oxidative damage. In this presentation, we describe the discovery and application of a novel function of selenite, that is, as a highly effective carrier to deliver iron for cell growth and function. In our in\u2010house\u2010developed animal protein\u2010free (APF) medium for CHO cells, using an iron\u2013selenite compound to replace the well\u2010established tropolone delivery system for iron led to comparable or better cell growth and antibody production. A high cell density of &gt;10\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> viable cells\/mL and excellent antibody titer of \u223c3 g\/L were achieved in 14\u2010day fed\u2010batch cultures in shake flasks, followed by successful scale\u2010up to stirred bioreactors. The preparation of the commercially unavailable iron\u2013selenite compound from respective ions, and its effectiveness in cell\u2010culture performance, were dependent on reaction time, substrates, and other conditions. \u00a9 2006 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.21081","type":"journal-article","created":{"date-parts":[[2006,8,25]],"date-time":"2006-08-25T22:56:14Z","timestamp":1156546574000},"page":"1188-1197","source":"Crossref","is-referenced-by-count":52,"title":["A novel function for selenium in biological system: Selenite as a highly effective iron carrier for Chinese hamster ovary cell growth and monoclonal antibody production"],"prefix":"10.1002","volume":"95","author":[{"given":"Jinyou","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Salmon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,8,25]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1536-4801.1998.tb01286.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0292-169"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1996.03540240035027"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.2307\/3284689"},{"key":"e_1_2_1_6_1","unstructured":"EMEA.2002.Note for guidance on minimizing the risk of transmitting animal spongiform encephalopathies via human and veterinary medicinal products. 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