{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T19:35:39Z","timestamp":1788809739245,"version":"build-2803163510"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,24]],"date-time":"2006-07-24T00:00:00Z","timestamp":1153699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp;amp; Bioengineering"],"published-print":{"date-parts":[[2007,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effect of nitrate, nitrite, nitric oxide (NO), and nitrous oxide on a mixed, mesophilic (35\u00b0C) methanogenic culture was investigated. Short\u2010term inhibition assays were conducted at a concentration range of 10\u2013350 mg N\/L nitrate, 17\u2013500 mg N\/L nitrite, 0.02\u20130.8 mg N\/L aqueous NO, and 19\u2013191 mg N\/L aqueous nitrous oxide. Simultaneous methane production and N\u2010oxide reduction was observed in 10 and 30 mg N\/L nitrate and 0.02 mg N\/L aqueous NO\u2010amended cultures. However, addition of N\u2010oxide resulted in immediate cessation of methanogenesis in all other cultures. Methanogenesis completely recovered subsequent to the complete reduction of N\u2010oxides to nitrogen gas in all N\u2010oxide\u2010amended cultures, with the exception of the 500 mg N\/L nitrite\u2010 and 0.8 mg N\/L aqueous NO\u2010amended cultures. Partial recovery of methanogenesis was observed in the 500 mg N\/L nitrite\u2010amended culture in contrast to complete inhibition of methanogenesis in the 0.8 mg N\/L aqueous NO\u2010amended culture. Accumulation of volatile fatty acids was observed in both cultures at the end of the incubation period. Among all N\u2010oxides, NO exerted the most and nitrate exerted the least inhibitory effect on the fermentative\/methanogenic consortia. The effect of multiple additions of nitrate (300 mg N\/L) on the same methanogenic culture was also investigated. Long\u2010term exposure of the methanogenic culture to nitrate resulted in an increase of N\u2010oxide reduction rates and decrease of methane production rates, which was attributed to changes in the microbial community structure due to nitrate addition. Biotechnol. Bioeng. 2007;96: 444\u2013455. \u00a9 2006 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.21105","type":"journal-article","created":{"date-parts":[[2006,7,24]],"date-time":"2006-07-24T21:46:28Z","timestamp":1153777588000},"page":"444-455","source":"Crossref","is-referenced-by-count":100,"title":["Inhibitory effects of nitrogen oxides on a mixed methanogenic culture"],"prefix":"10.1002","volume":"96","author":[{"given":"A. 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