{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T17:39:03Z","timestamp":1787593143755,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,12,14]],"date-time":"2006-12-14T00:00:00Z","timestamp":1166054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Polyketides are a group of natural products that have gained much interest due to their use as antibiotics, cholesterol lowering agents, immunosuppressors, and as other drugs. Many organisms that naturally produce polyketides are difficult to cultivate and only produce these metabolites in small amounts. It is therefore of general interest to transfer polyketide synthase (PKS) genes from their natural sources into heterologous hosts that can over\u2010produce the corresponding polyketides. In this study we demonstrate the heterologous expression of 6\u2010methylsalicylic acid synthase (6\u2010MSAS), naturally produced by <jats:italic>Penicillium patulum<\/jats:italic>, in the yeast <jats:italic>Saccharomyces cerevisiae<\/jats:italic>. In order to activate the PKS a 4\u2032\u2010phosphopantetheinyl transferase (PPTase) is required. We therefore co\u2010expressed PPTases encoded by either <jats:italic>sfp<\/jats:italic> from <jats:italic>Bacillus subtilis<\/jats:italic> or by <jats:italic>npgA<\/jats:italic> from <jats:italic>Aspergillus nidulans<\/jats:italic>. The different strains were grown in batch cultures. Growth and product concentration were measured and kinetic parameters were calculated. It was shown that both PPTases could be efficiently used for activation of PKS's in yeast as good yields of 6\u2010MSA were obtained with both enzymes. Biotechnol. Bioeng. 2007;97: 893\u2013900. \u00a9 2006 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.21286","type":"journal-article","created":{"date-parts":[[2006,12,14]],"date-time":"2006-12-14T22:17:44Z","timestamp":1166134664000},"page":"893-900","source":"Crossref","is-referenced-by-count":53,"title":["Optimization of heterologous production of the polyketide 6\u2010MSA in <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1002","volume":"97","author":[{"given":"Songsak","family":"Wattanachaisaereekul","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Eliasson","family":"Lantz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael Lynge","family":"Nielsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d3lafur 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