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Because no effective vaccine has yet been established, we designed a new biomolecule as a vaccination antigen capable of preventing the infection of <jats:italic>H. pylori<\/jats:italic>. The designed biomolecule involves a 138 stretch (aa 201\u2013aa 338 of \u03b2\u2010subunit of <jats:italic>H. pylori<\/jats:italic> urease), which is the functionally important region for urease activity. The region was expressed as a recombinant protein, called UREB138. The therapeutic vaccination was performed using UREB138 in mice persistently infected with <jats:italic>H. pylori<\/jats:italic>. The subcutaneous administration of UREB138 remarkably reduced the number of bacteria in the mice stomach compared with the control. Immunization with UREB138 enhanced the urease\u2010specific IgA and IgG1 in the serum. Immunohistochemical staining for IgA in gastric mucosa showed that the number of mice positively stained with IgA was significantly higher in UREB138\u2010immunized mice than in control mice. Furthermore, the expression of interferon\u2010gamma mRNA in the gastric tissues with eradicated bacteria was higher than in the non\u2010eradicated group. The combination of Th1\u2010 and Th2\u2010mediated immunity plays a role in reducing the colonization of bacterial numbers of <jats:italic>H. pylori<\/jats:italic>. Biotechnol. Bioeng. 2008;100: 634\u2013643. \u00a9 2008 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.21804","type":"journal-article","created":{"date-parts":[[2008,2,7]],"date-time":"2008-02-07T10:22:39Z","timestamp":1202379759000},"page":"634-643","source":"Crossref","is-referenced-by-count":6,"title":["Therapeutic effects of molecularly designed antigen UREB138 for mice infected with <i>Helicobacter 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