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Glucose removal was higher from all pretreatments than from Avicel cellulose at lower enzyme loadings, but sugar release was a bit lower for solids prepared by dilute sulfuric acid in the Sunds system and by controlled pH pretreatment than from Avicel at higher protein loadings. Inhibition by cellobiose was observed to depend on the type of substrate and pretreatment and hydrolysis times, with a corresponding impact of \u03b2\u2010glucosidase supplementation. Furthermore, for the first time, xylobiose and higher xylooligomers were shown to inhibit enzymatic hydrolysis of pure glucan, pure xylan, and pretreated corn stover, and xylose, xylobiose, and xylotriose were shown to have progressively greater effects on hydrolysis rates. Consistent with this, addition of xylanase and \u03b2\u2010xylosidase improved performance significantly. For a combined mass loading of cellulase and \u03b2\u2010glucosidase of 16.1 mg\/g original glucan (about 7.5 FPU\/g), glucose release from pretreated solids ranged from 50% to75% of the theoretical maximum and was greater for all pretreatments at all protein loadings compared to pure Avicel cellulose except for solids from controlled pH pretreatment and from dilute acid pretreatment by the Sunds pilot unit. The fraction of xylose released from pretreated solids was always less than for glucose, with the upper limit being about 60% of the maximum for ARP and the Sunds dilute acid pretreatments at a very high protein mass loading of 116 mg\/g glucan (about 60 FPU). Biotechnol. Bioeng. 2009;102: 457\u2013467. \u00a9 2008 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.22068","type":"journal-article","created":{"date-parts":[[2008,7,25]],"date-time":"2008-07-25T17:53:21Z","timestamp":1217008401000},"page":"457-467","source":"Crossref","is-referenced-by-count":177,"title":["Effect of enzyme supplementation at moderate cellulase loadings on initial glucose and xylose release from corn stover solids pretreated by leading 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