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The toxin belongs to a family of superantigens (SAgs) which exhibit several shared biological properties, including the induction of massive cytokine release and V<jats:sub>\u03b2<\/jats:sub>\u2010specific T\u2010cell proliferation. In this study we explored the possibility to use monoclonal Variable domains of Llama Heavy\u2010chain antibodies (VHH) in the immuno capturing of TSST\u20101 from plasma. Data is presented that the selected VHHs are highly specific for TSST\u20101 and can be efficiently produced in large amounts in yeast. In view of affinity chromatography, the VHHs are easily coupled to beads, and are able to deplete TSST\u20101 from plasma at very low, for example, pathologically relevant, concentrations. When spiked with 4\u2009ng\/mL TSST\u20101 more than 96% of TSST\u20101 was depleted from pig plasma. These data pave the way to further explore application of high\u2010affinity columns in the specific immuno depletion of SAgs in experimental sepsis models and in sepsis in humans. Biotechnol. Bioeng. 2009; 104: 143\u2013151 \u00a9 2009 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.22365","type":"journal-article","created":{"date-parts":[[2009,4,13]],"date-time":"2009-04-13T20:17:11Z","timestamp":1239653831000},"page":"143-151","source":"Crossref","is-referenced-by-count":23,"title":["Specific immuno capturing of the Staphylococcal superantigen toxic\u2010shock syndrome toxin\u20101 in plasma"],"prefix":"10.1002","volume":"104","author":[{"given":"Hendrik","family":"Adams","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter","family":"Brummelhuis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bram","family":"Maassen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"van Egmond","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed 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