{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T04:07:58Z","timestamp":1773202078962,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,5,28]],"date-time":"2009-05-28T00:00:00Z","timestamp":1243468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A complete set of mathematically identifiable and meaningful kinetic parameters estimates is needed to accurately describe the activity of individual populations that dehalorespire tetrachloroethene (PCE) and other chlorinated ethenes. These data may be difficult to extract from the literature because kinetic parameter estimates obtained using mixed cultures may reflect the activity of multiple dehalorespiring populations, while those obtained at low initial substrate\u2010to\u2010biomass ratios (<jats:italic>S<\/jats:italic><jats:sub>0<\/jats:sub>\/<jats:italic>X<\/jats:italic><jats:sub>0<\/jats:sub>) are influenced by culture history and are generally not relevant to other systems. This study focused on estimation of electron donor and acceptor utilization kinetic parameters for the heterotrophic dehalorespirers <jats:italic>Desulfuromonas michiganensis<\/jats:italic> strain BB1 and <jats:italic>Desulfitobacterium<\/jats:italic> sp. strain PCE1. Electron acceptor utilization kinetic parameters that are identifiable and independent of culture history, i.e., intrinsic, could be estimated at <jats:italic>S<\/jats:italic><jats:sub>0<\/jats:sub>\/<jats:italic>X<\/jats:italic><jats:sub>0<\/jats:sub>\u2009\u2265\u200910, with both concentrations expressed as chemical oxygen demand (COD). However, the parameter estimates did not accurately describe dechlorination kinetics at lower <jats:italic>S<\/jats:italic><jats:sub>0<\/jats:sub>\/<jats:italic>X<\/jats:italic><jats:sub>0<\/jats:sub> ratios. The maximum specific substrate utilization rates (<jats:italic>q<\/jats:italic><jats:sub>max<\/jats:sub>) and half\u2010saturation constants (<jats:italic>K<\/jats:italic><jats:sub><jats:italic>S<\/jats:italic><\/jats:sub>) for PCE and trichloroethene (TCE) estimated for the two heterotrophic strains are higher than the values reported for <jats:italic>Dehalococcoides<\/jats:italic> cultures. These results suggest that the natural niche of <jats:italic>Dehalococcoides<\/jats:italic> strains that can metabolize a range of chlorinated ethenes may be to respire dichloroethene and vinyl chloride produced by <jats:italic>Desulfuromonas<\/jats:italic> and <jats:italic>Desulfitobacterium<\/jats:italic> strains or other populations that dechlorinate PCE and TCE at faster rates. Few data exist on the electron donor utilization kinetics of heterotrophic dehalorespirers. The results of this study suggest that <jats:italic>Desulfuromonas<\/jats:italic> and <jats:italic>Desulfitobacterium<\/jats:italic> strains should be able to compete for organic electron donors with other heterotrophic populations in the subsurface. Biotechnol. Bioeng. 2009; 104: 301\u2013311 \u00a9 2009 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.22421","type":"journal-article","created":{"date-parts":[[2009,5,28]],"date-time":"2009-05-28T10:40:00Z","timestamp":1243507200000},"page":"301-311","source":"Crossref","is-referenced-by-count":22,"title":["Determination of intrinsic monod kinetic parameters for two heterotrophic tetrachloroethene (PCE)\u2010respiring strains and insight into their application"],"prefix":"10.1002","volume":"104","author":[{"given":"Deyang","family":"Huang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer G.","family":"Becker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,7,10]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/es061438n"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1061\/(ASCE)0733-9372(1998)124:11(1076)"},{"issue":"14","key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"4473","DOI":"10.1021\/es051849o","article-title":"A modeling study and implications of competition between Dehalococcoides ethenogenes and other tetrachloroethene\u2010respiring bacteria","volume":"40","author":"Becker JG","year":"2006","journal-title":"Environ Sci Technol"},{"issue":"3","key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1021\/es801616f","article-title":"Modeling the effects of microbial competition and hydrodynamics on the dissolution and detoxification of dense nonaqueous phase liquid contaminants","volume":"43","author":"Becker JG","year":"2009","journal-title":"Environ Sci Technol"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21932"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1354(02)00104-5"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-007-1097-3"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00411655"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.2.953-959.2003"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/es0348647"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.2175\/106143096X127929"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2005.00731.x"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/es980136l"},{"issue":"12","key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"5212","DOI":"10.1128\/AEM.65.12.5212-5221.1999","article-title":"Influence of different electron donors and acceptors on dehalorespiration of tetrachloroethene by Desulfitobacterium frappieri TCE1","volume":"65","author":"Gerritse J","year":"1999","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050308"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/es00156a012"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0043-1354(95)00199-9"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/es9805876"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.2.996-1003.2003"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1038\/nature01717","article-title":"Detoxification of vinyl chloride to ethene coupled to growth of an anaerobic bacterium","volume":"424","author":"He J","year":"2003","journal-title":"Nature"},{"issue":"11","key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"6940","DOI":"10.1128\/AEM.70.11.6940-6943.2004","article-title":"Acetate threshold concentrations suggest varying energy requirements during anaerobic respiration by Anaeromyxobacter dehalogenans","volume":"70","author":"He Q","year":"2004","journal-title":"Appl Environ Microbiol"},{"issue":"2","key":"e_1_2_1_23_1","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1128\/aem.61.2.526-530.1995","article-title":"Evidence for an anaerobic syntrophic benzoate degradation threshold and isolation of the syntrophic benzoate degrader","volume":"61","author":"Hopkins BT","year":"1995","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1990.tb03958.x"},{"key":"e_1_2_1_25_1","unstructured":"Krajmalnik\u2010BrownR.2005.Genetic identification of reductive dehalogenase genes inDehalococcoides. 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