{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T10:27:41Z","timestamp":1788776861910,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T00:00:00Z","timestamp":1246406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp;amp; Bioengineering"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Klebsiella pneumoniae<\/jats:italic> HR526, a new isolated 1,3\u2010propanediol (1,3\u2010PD) producer, exhibited great productivity. However, the accumulation of lactate in the late\u2010exponential phase remained an obstacle of 1,3\u2010PD industrial scale production. Hereby, mutants lacking <jats:sc>D<\/jats:sc>\u2010lactate pathway were constructed by knocking out the <jats:italic>ldh<\/jats:italic>A gene encoding fermentative <jats:sc>D<\/jats:sc>\u2010lactate dehydrogenase (LDH) of HR526. The mutant <jats:italic>K. pneumoniae<\/jats:italic> LDH526 with the lowest LDH activity was studied in aerobic fed\u2010batch fermentation. In experiments using pure glycerol as feedstock, the 1,3\u2010PD concentrations, conversion, and productivity increased from 95.39\u2009g\u2009L<jats:sup>\u22121<\/jats:sup>, 0.48 and 1.98\u2009g\u2009L<jats:sup>\u22121<\/jats:sup>\u2009h<jats:sup>\u22121<\/jats:sup> to 102. 06\u2009g\u2009L<jats:sup>\u22121<\/jats:sup>, 0.52\u2009mol\u2009mol<jats:sup>\u22121<\/jats:sup> and 2.13\u2009g\u2009L<jats:sup>\u22121<\/jats:sup>\u2009h<jats:sup>\u22121<\/jats:sup>, respectively. The diol (1,3\u2010PD and 2,3\u2010butanediol) conversion increased from 0.55\u2009mol\u2009mol<jats:sup>\u22121<\/jats:sup> to a maximum of 0.65\u2009mol\u2009mol<jats:sup>\u22121<\/jats:sup>. Lactate would not accumulate until 1,3\u2010PD exceeded 84\u2009g\u2009L<jats:sup>\u22121<\/jats:sup>, and the final lactate concentration decreased dramatically from more than 40\u2009g\u2009L<jats:sup>\u22121<\/jats:sup> to &lt;3\u2009g\u2009L<jats:sup>\u22121<\/jats:sup>. Enzymic measurements showed LDH activity decreased by 89\u201398% during fed\u2010batch fermentation, and other related enzyme activities were not affected. NADH\/NAD<jats:sup>+<\/jats:sup> enhanced more than 50% in the late\u2010exponential phase as the <jats:sc>D<\/jats:sc>\u2010lactate pathway was cut off, which might be the main reason for the change of final metabolites concentrations. The ability to utilize crude glycerol from biodiesel process and great genetic stability demonstrated that <jats:italic>K. pnemoniae<\/jats:italic> LDH526 was valuable for 1,3\u2010PD industrial production. Biotechnol. Bioeng. 2009; 104: 965\u2013972. \u00a9 2009 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.22455","type":"journal-article","created":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T17:07:29Z","timestamp":1246468049000},"page":"965-972","source":"Crossref","is-referenced-by-count":117,"title":["Metabolism in 1,3\u2010propanediol fed\u2010batch fermentation by a <scp>D<\/scp>\u2010lactate deficient mutant of <i>Klebsiella pneumoniae<\/i>"],"prefix":"10.1002","volume":"104","author":[{"given":"Yun\u2010Zhen","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ni\u2010Ni","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zong\u2010Ming","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian\u2010Jin","family":"Ou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Juan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De\u2010Hua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,7,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19980905)59:5<544::AID-BIT3>3.0.CO;2-A"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/BF00164474","article-title":"Fermentation 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