{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:05:46Z","timestamp":1790978746128,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,10,26]],"date-time":"2009-10-26T00:00:00Z","timestamp":1256515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp;amp; Bioengineering"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We developed an alternative patterning technique which is capable of producing both topographic and biochemical features for cell culture studies. This technique is based on microaspiration induced with a degassed poly (dimethylsiloxane) (PDMS) mold. After degassing in a rough vacuum chamber and placed on a sample surface, liquid solution can be aspired through channels and cavities created in the PDMS mold. Depending on the composition of the solution and the associated drying or incubation processes, a variety of surface patterns can be produced without applying external pressure. For demonstration, we fabricated agarose gel microwells and biomolecule patterns either on a glass plate or in a cell culture Petri dish, both applicable for cell culture studies. Biotechnol. Bioeng. 2010. 105: 854\u2013859. \u00a9 2009 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.22586","type":"journal-article","created":{"date-parts":[[2009,10,26]],"date-time":"2009-10-26T22:28:09Z","timestamp":1256596089000},"page":"854-859","source":"Crossref","is-referenced-by-count":20,"title":["Degassing\u2010assisted patterning of cell culture surfaces"],"prefix":"10.1002","volume":"105","author":[{"given":"Chunxiong","family":"Luo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofang","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin\u2010ichiro M.","family":"Nomura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,12,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10544-008-9167-6"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.040562297"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mee.2006.01.015"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5313.779"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/b403930k"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/b513424b"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/B810341K"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4095(199908)11:11<946::AID-ADMA946>3.0.CO;2-9"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/b508096g"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.10.060"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/b204944a"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10544-007-9066-2"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21877"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1002\/(SICI)1099-0488(20000201)38:3<415::AID-POLB8>3.0.CO;2-Z","article-title":"Gas sorption, diffusion and permeation in poly(dimethylsiloxane)","volume":"38","author":"Merkel TC","year":"2000","journal-title":"Polym Phys"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.2116\/analsci.24.67"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.200501554"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/b613349e"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"11377","DOI":"10.1021\/la702076u","article-title":"Patterning biomolecules with a water\u2010soluble release and protection interlayer","volume":"23","author":"Shi J","year":"2007","journal-title":"Langmuir"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8171320"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac050131o"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(03)00543-X"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsusc.2005.11.091"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac070306p"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.22586","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.22586","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T14:15:29Z","timestamp":1759932929000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.22586"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12,18]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2010,3]]}},"alternative-id":["10.1002\/bit.22586"],"URL":"https:\/\/doi.org\/10.1002\/bit.22586","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,12,18]]}}}