{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:32:08Z","timestamp":1786977128428,"version":"build-2736575974"},"reference-count":56,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,4,16]],"date-time":"2010-04-16T00:00:00Z","timestamp":1271376000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2010,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Glial\u2010derived neurotrophic factor (GDNF) and nerve growth factor (NGF) have both been shown to enhance peripheral nerve regeneration following injury and target different neuronal populations. The delivery of either growth factor at the site of injury may, therefore, result in quantitative differences in motor nerve regeneration and functional recovery. In this study we evaluated the effect of affinity\u2010based delivery of GDNF or NGF from fibrin\u2010filled nerve guidance conduits (NGCs) on motor nerve regeneration and functional recovery in a 13\u2009mm rat sciatic nerve defect. Seven experimental groups were evaluated consisting of GDNF or NGF and the affinity\u2010based delivery system (DS) within NGCs, control groups excluding the DS and\/or growth factor, and nerve isografts. Groups with growth factor in the conduit demonstrated equivalent or superior performance in behavioral tests and relative muscle mass measurements compared to isografts at 12 weeks. Additionally, groups with GDNF demonstrated greater specific twitch and tetanic force production in extensor digitorum longus (EDL) muscle than the isograft control, while groups with NGF produced demonstrated similar force production compared to the isograft control. Assessment of motor axon regeneration by retrograde labeling further revealed that the number of ventral horn neurons regenerating across NGCs containing GDNF and NGF DS was similar to the isograft group and these counts were greater than the groups without growth factor. Overall, the GDNF DS group demonstrated superior functional recovery and equivalent motor nerve regeneration compared to the isograft control, suggesting it has potential as a treatment for motor nerve injury. Biotechnol. Bioeng. 2010;106: 970\u2013979. \u00a9 2010 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.22766","type":"journal-article","created":{"date-parts":[[2010,4,16]],"date-time":"2010-04-16T12:21:31Z","timestamp":1271420491000},"page":"970-979","source":"Crossref","is-referenced-by-count":79,"title":["Fibrin matrices with affinity\u2010based delivery systems and neurotrophic factors promote functional nerve regeneration"],"prefix":"10.1002","volume":"106","author":[{"given":"Matthew D.","family":"Wood","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew R.","family":"MacEwan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander R.","family":"French","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy M.","family":"Moore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Hunter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan E.","family":"Mackinnon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel W.","family":"Moran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory H.","family":"Borschel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelly E.","family":"Sakiyama\u2010Elbert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,6,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.10.3519"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.7.2795"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.PRS.0000118049.93654.CA"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-198901000-00024"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10434"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.20127"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1097\/00003086-198409000-00029","article-title":"Results of nerve grafting in severe soft tissue injuries","volume":"188","author":"Beazley WC","year":"1984","journal-title":"Clin Orthop Relat Res"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1385\/MN:27:3:277"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(82)90234-5"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0266-7681(94)90067-1"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-198811000-00020"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.08.045"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2002.01892.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.1998.19320195.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/039139880402700208"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-199202000-00009"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000637-199302000-00009"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19960212)365:3<380::AID-CNE4>3.0.CO;2-0"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1620-06.2006"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(83)80645-0"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03290.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.32343"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.22.7216"},{"issue":"1","key":"e_1_2_6_25_1","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1182\/blood.V66.1.157.157","article-title":"Acceleration of fibrinolysis by the N\u2010terminal peptide of alpha 2\u2010plasmin inhibitor","volume":"66","author":"Kimura S","year":"1985","journal-title":"Blood"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.98.2.773"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4886(03)00258-9"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hcl.2008.08.001"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-198503000-00014"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.2006.01657.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.20155"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.1920100213"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)02778-5"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-0102(03)00010-5"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1159\/000111912"},{"issue":"12","key":"e_1_2_6_36_1","doi-asserted-by":"crossref","first-page":"4269","DOI":"10.1523\/JNEUROSCI.09-12-04269.1989","article-title":"Neuronal plasminogen activators: Cell surface binding sites and involvement in neurite outgrowth","volume":"9","author":"Pittman RN","year":"1989","journal-title":"J Neurosci"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2004.08.007"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.13.15.2214"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-3659(00)00296-0"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-3659(99)00221-7"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc9800769"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bioeng.5.011303.120731"},{"issue":"2","key":"e_1_2_6_43_1","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1523\/JNEUROSCI.08-02-00664.1988","article-title":"Expression of nerve growth factor receptors by Schwann cells of axotomized peripheral nerves: Ultrastructural location, suppression by axonal contact, and binding properties","volume":"8","author":"Taniuchi M","year":"1988","journal-title":"J Neurosci"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi952532b"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20533"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560030410"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1996.0448"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-1000085"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.21220"},{"issue":"3","key":"e_1_2_6_50_1","first-page":"505","article-title":"Some additional parametric variations between peripheral nerve fibre populations","volume":"109","author":"Williams PL","year":"1971","journal-title":"J Anat"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.31269"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.32043"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2008.11.008"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1163\/156856209X445285"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.52.070183.003553"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.06.067"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2004.21.1468"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.22766","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.22766","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T18:28:47Z","timestamp":1759948127000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.22766"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6,29]]},"references-count":56,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,8,15]]}},"alternative-id":["10.1002\/bit.22766"],"URL":"https:\/\/doi.org\/10.1002\/bit.22766","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,6,29]]}}}