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The reductively dechlorinating anaerobic Evanite subculture (EV\u2010cDCE) was fed trichloroethene (TCE) and excess electron donor to accumulate <jats:italic>cis<\/jats:italic>\u20101,2\u2010dichloroethene (cDCE) in a continuous flow stirred tank reactor (CFSTR); and an attached growth recirculating packed column (RPC). A concentration\u2010dependent toxicity model used to simulate the results of batch reactors in part I (Sabalowsky and Semprini (in press) Biotechnol Bioeng) also simulated well the observations for the CFSTR and RPC growth modes. The toxicity model incorporates cDCE and TCE toxicity coefficients that directly increase the cell decay coefficient in proportion with cDCE and TCE concentrations. Simulated estimates of the cDCE and TCE toxicity coefficients indicate reductively dechlorinating cells are most sensitive to high concentrations of cDCE and TCE in batch\u2010fed growth, followed by CFSTR, with attached growth being least sensitive. The greater toxicity of TCE than cDCE, and ratio of the modeled toxicity coefficients, agrees with previously proposed models relating toxicity to partitioning in the cell wall (<jats:italic>K<\/jats:italic><jats:sub>M\/B<\/jats:sub>), proportional to octanol\u2010water partitioning (<jats:italic>K<\/jats:italic><jats:sub>OW<\/jats:sub>) coefficients. Biotechnol. Bioeng. 2010;107: 540\u2013549. \u00a9 2010 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.22822","type":"journal-article","created":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T20:33:19Z","timestamp":1275424399000},"page":"540-549","source":"Crossref","is-referenced-by-count":12,"title":["Trichloroethene and <i>cis<\/i>\u20101,2\u2010dichloroethene concentration\u2010dependent toxicity model simulates anaerobic dechlorination at high concentrations. 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