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Here, we report development of a metabolically engineered strain of <jats:italic>Escherichia coli<\/jats:italic> that overproduces cadaverine in glucose mineral salts medium. First, cadaverine degradation and utilization pathways were inactivated. Next, <jats:sc>L<\/jats:sc>\u2010lysine decarboxylase, which converts <jats:sc>L<\/jats:sc>\u2010lysine directly to cadaverine, was amplified by plasmid\u2010based overexpression of the <jats:italic>cadA<\/jats:italic> gene under the strong <jats:italic>tac<\/jats:italic> promoter. Furthermore, the <jats:sc>L<\/jats:sc>\u2010lysine biosynthetic pool was increased by the overexpression of the <jats:italic>dapA<\/jats:italic> gene encoding dihydrodipicolinate synthase through the replacement of the native promoter with the strong <jats:italic>trc<\/jats:italic> promoter in the genome. The final engineered strain was able to produce 9.61\u2009g\u2009L<jats:sup>\u22121<\/jats:sup> of cadaverine with a productivity of 0.32\u2009g\u2009L<jats:sup>\u22121<\/jats:sup>\u2009h<jats:sup>\u22121<\/jats:sup> by fed\u2010batch cultivation. The strategy reported here should be useful for the bio\u2010based production of cadaverine from renewable resources. Biotechnol. Bioeng. 2011; 108:93\u2013103. \u00a9 2010 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.22918","type":"journal-article","created":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T13:43:20Z","timestamp":1283348600000},"page":"93-103","source":"Crossref","is-referenced-by-count":213,"title":["Metabolic engineering of <i>Escherichia coli<\/i> for the production of cadaverine: A five carbon diamine"],"prefix":"10.1002","volume":"108","author":[{"given":"Zhi\u2010Gang","family":"Qian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Xia","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Yup","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,9,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2004.tb09577.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06450"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-60-2-159"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"12419","DOI":"10.1016\/S0021-9258(19)86331-7","article-title":"Mutants of Escherichia coli that do not contain 1,4\u2010diaminobutane (putrescine) or spermidine","volume":"254","author":"Hafner EW","year":"1979","journal-title":"J Biol Chem"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1246\/cl.1986.893"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1985.tb08836.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1010"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.22005"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21433"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00981.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2010.03.005"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M800133200"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7799(96)80930-9"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00131766"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/msb4100196"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.8.2659-2669.1992"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.60699"},{"key":"e_1_2_6_20_1","unstructured":"NishiK EndoS MoriY TotsukaK HiraoY.2007. 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